Infertility
Conditions
Keywords
Vitamin D, 25(OH)D, in vitro fertilization, AMH, infertility, clinical pregnancy
Brief summary
Non skeletal effect of vitamin D have been the focus of much interest in the past decade. During the last years, special attention has been given to the impact of vitamin D on human reproduction. Its receptor is expressed in most reproductive organs including hypothalamus, pituitary gland, uterus, ovary, mammary gland and the placenta. In vitro experiments suggest that vitamin D would be involved in regulated embryo-implantation. Concerning AMH, vitamin D seems to alters AMH signaling in human granulosa cells although their circulated levels are correlated in lated reproductive aged women. The results of precedents studies about the impact of vitamin D on IVF outcomes remain conflicting The aim of our study was to assess in a large unselected population of infertile women, the impact of vitamin D deficiency on IVF outcome and to examine the relationship between serum vitamin D levels and the ovarian reserve marker AMH.
Detailed description
Vitamin D was measured on the sixth day of stimulation. According to vitamin D level, women were divided into 2 groups (deficiency when 25(OH)D \<20 ng/ml and sufficient when 25(OH)D \>20 ng/ml). Pregnancy rates were compared among these two groups after controlling age, BMI, characteristics of ovarian stimulation, number of ovocytes obtains, etc.
Interventions
BIOLOGICALvitamin D estimation
Serum samples to estimate vitamin D status were collected on the sixth day of stimulation
Sponsors
Nantes University Hospital
Study design
Observational model
CASE_CONTROL
Time perspective
RETROSPECTIVE
Eligibility
Sex/Gender
FEMALE
Age
18 Years to 42 Years
Healthy volunteers
No
Inclusion criteria
* Patients undergoing IVF/ICSI in the center of medical assistance for the procreation of the french hospital of Nantes between september 2010 and may 2011.
Exclusion criteria
* Women for who the cycle was interrupted during ovarian stimulation or converted in intrauterine insemination.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Laboratory pregnancy | 4 weeks to 9 month | Serum HCG was drawn 16 days after ovocyte tapping; Patient had a repeat bHCG drawn 48 hours if they have a positive result from the first bHCG; Clinical pregnancy was defined as ultrasound documentation of fetal heart tones at seven weeks of gestation |
| Clinical pregnancy | 4 weeks to 9 month | Serum HCG was drawn 16 days after ovocyte tapping; Patient had a repeat bHCG drawn 48 hours if they have a positive result from the first bHCG; Clinical pregnancy was defined as ultrasound documentation of fetal heart tones at seven weeks of gestation |
| Live birth | 4 weeks to 9 month | Serum HCG was drawn 16 days after ovocyte tapping; Patient had a repeat bHCG drawn 48 hours if they have a positive result from the first bHCG; Clinical pregnancy was defined as ultrasound documentation of fetal heart tones at seven weeks of gestation |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| The relationship between serum vitamin D levels and the ovarian reserve marker AMH | day 3 | Serum AMH was drawn during hormonal examination on third day of natural cycle. |
Contacts
PRINCIPAL_INVESTIGATORSophie DUBOURDIEU, Dr
Nantes University Hospital
Outcome results
None listed