Acute Exercise, Cardiovascular Diseases, Obesity, Type 2 Diabetes
Conditions
Brief summary
The purpose of this study is to determine the ability of acute exercise to regulate fat metabolism in muscle of overweight and obese people compared to lean people.
Detailed description
After being informed about the study details and possible risks and benefits, 15 overweight/obese and 15 lean participants gave written informed consent and were then screened and enrolled in the study. All participants then gave a muscle sample and ate a calorically controlled breakfast. Participants completed \ 1 hour of cycling exercise on a stationary bike and gave a second muscle sample 4 hours after eating breakfast.
Interventions
BEHAVIORALAcute exercise
All participants consumed a standardized, high carbohydrate meal (7 kcal/kg; 60% carbohydrate, 25% fat, 15% protein) and completed an exercise testing trial, expending a total of 650 kcal upon completion 4h later.
Sponsors
Purdue University
Louisiana State University Health Sciences Center Shreveport
East Carolina University
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
SINGLE (Outcomes Assessor)
Masking description
Investigators conducting molecular analysis were blinded to study samples/groups.
Intervention model description
All participants underwent the same exact study protocol on random days throughout the study period.
Eligibility
Sex/Gender
MALE
Age
18 Years to 30 Years
Healthy volunteers
Yes
Inclusion criteria
* healthy males that completed a physical examination with a State Licensed M.D. or D.O. within the past 2 years and completed a physical activity readiness questionnaire (PARQ), the International Physical Activity Questionnaires (IPAQ) and and a medical history questionnaire prior to the study
Exclusion criteria
* any participants that had not had a physical examination by a State Licensed M.D. or D.O. within the past 2 years * persons not considered healthy (with any previous medical diagnoses) or not medically cleared by a M.D. or D.O. to perform physical activity/exercise * persons who are regularly physically active
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Change from baseline in skeletal muscle acylcarnitines | Baseline and immediately after acute cycling exercise | Metabolomics of skeletal muscle acylcarnitines is used as a measure of mitochondrial function by assessing long, medium and short chain acylcarnitine levels. |
| Change from baseline in skeletal transcriptome | Baseline and immediately after acute cycling exercise | RNA-seq is a method used to quantify changes in all known messenger RNA levels in the genome, giving an assessment of whole genome gene transcription changes. |
| Change from baseline in skeletal muscle whole genome nucleosome maps | Baseline and immediately after acute cycling exercise | MNase-seq is a method used to determine the occupancy/location of nucleosomes throughout the entire genome, supporting evidence of alterations in epigenetic modifications occurring at specific genomic loci that affect nucleosome occupancy/location and possibly gene transcription or silencing. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Free fatty acids | Baseline and immediately after acute cycling exercise | Commercially validated and available colorimetric kits used to determine plasma free fatty acid levels. |
| Glucose | Baseline and immediately after acute cycling exercise | Commercially validated and available colorimetric used to determine plasma glucose levels. |
| Cholesterol | Baseline and immediately after acute cycling exercise | Commercially validated and available colorimetric kits used to determine total cholesterol, HDL, LDL and VLDL levels in plasma. |
| Insulin | Baseline and immediately after acute cycling exercise | Commercially validated and available ELISAs used to determine plasma insulin levels. |
| Triglycerides | Baseline and immediately after acute cycling exercise | Commercially validated and available colorimetric kits used to determine plasma triglyceride levels. |
Countries
United States
Outcome results
None listed