Changes in Bacterial Count in Axilla After Laser Hair Removal
Conditions
Brief summary
1. To evaluate the effect of ND-YAG laser hair removal on some skin flora and pathogens: an in vivo study. 2. To determine the immediate and long term effect of ND-YAG laser on total viable bacterial count, some skin flora and pathogens of laser-assisted hair removal in axilla. 3. To assess the relation between this effect and bromohidrosis.
Detailed description
This Quasi-experimental interrupted time series study will be conducted on 30 female participants who will be recruited from the Dermatology Outpatient Clinic of the Alexandria Main University Hospital. Four sessions of laser assisted hair removal at 4 weeks intervals (S1, S2, S3 and S4) using long pulsed ND: YAG (Cynosure Elite+™, Westford, USA). The following parameters will be used according to the nature of hair (1064 nm, 15-18 mm spot size, fluence of 24-35 J/cm2, pulse duration 30-40 ms). 4\. Swabs will be collected immediately and after each session
Interventions
laser hair removal sessions
Sponsors
Study design
Eligibility
Inclusion criteria
No previous laser hair removal sessions involving axilla. Fitzpatrick skin type III-VI.
Exclusion criteria
1. Skin diseases such as atopic dermatitis, psoriasis, fungal or herpetic infections. 2. Systemic diseases such as diabetes or endocrinal dysfunction. 3. Patients receiving immunosuppressive therapy. 4. Pregnant or lactating females.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| to compare changes in bacterial count and composition of axillary flora after laser hair removal | 4 month | counting bacterial colonies (aerobic,anaerobic,lipophyllic and staphylococcal) using colony counter |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| To determine the immediate and long term effect of ND-YAG laser on total viable bacterial count, some skin flora and pathogens of laser-assisted hair removal in axilla | 4 month | counting bacterial colonies immediately before and immediately after each laser session |
| To assess the relation between this effect and bromohidrosis. | 4 month | using a questionnaire that is categorized into better , worse or unchanged sweat odour. |
Countries
Egypt