Muscle Damage
Conditions
Keywords
Exercise-induced muscle damage, Recovery, Supplementation, Oxidative stress, Anthocyanin, New Zealand blackcurrant
Brief summary
The study aims to examine the effect of a New Zealand blackcurrant (NZBC) supplement on markers of muscle damage and recovery following strenuous resistance exercise. The investigation will compare responses between an experimental (NZBC capsule, 300 mg/day) and placebo (PLA capsule, 300 mg sugar) group. Participants will attend a screening session where they will consent to the study, complete a pre-activity medical questionnaire and have their height, weight and resting blood pressure measured. If the participants meet the inclusion criteria they will perform a familarisation session on the muscle strength assessment. Participants will be randomized to NZBC or placebo groups, and consume one capsule in the morning (between 6-10 am) for 12 days. This is a double-blinded study, which means that the participant and the study team will not know which group the participants are assigned to until the study is over. On day 8 participants will perform a strenuous bout of upper body resistance exercise on the isokinetic dynamometer (exercise device). Muscle strength and soreness, arm circumference, and elbow range of motion will be measured, and a fasted blood sample will be collected, before and 24, 48, 72 & 96 hours after the muscle fatigue protocol (on days 9, 10, 11 & 12). A marker of muscle damage (creatine kinase \[CK\] concentration) will be measured in the blood samples. Participants will also be asked to complete a 6-day dietary record, beginning on day 7 and ending on the final day of testing (day 12).
Interventions
DIETARY_SUPPLEMENTNew Zealand blackcurrants (NZBC)
NZBC capsules containing anthocyanin-rich blackcurrant extract
DIETARY_SUPPLEMENTPlacebo (PLA)
Placebo capsules containing microcrystalline cellulose
Sponsors
University of Surrey
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
PREVENTION
Masking
DOUBLE (Subject, Investigator)
Masking description
Coded, capsules
Intervention model description
Randomised, parallel design.
Eligibility
Sex/Gender
ALL
Age
18 Years to 45 Years
Healthy volunteers
Yes
Inclusion criteria
* Males and Females * 18 - 45 years old * BMI: 19 - 29.9 kg/m2 * Healthy: no active disease process that could interfere with endpoints measured as determined by medical history
Exclusion criteria
* Smoking and tobacco use * Takes medication \[excluding contraception\] * BMI ≥ 30 kg/m2 * Hypertensive (diastolic \> 90 and/or systolic blood pressure \> 140 mmHg) * History of musculoskeletal upper limb injuries * Performs regular resistance exercise (\> 2 sessions per week) * Uses dietary supplements that could influence muscle recovery or function (e.g. protein supplements, antioxidants etc)
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Change from baseline in creatine kinase (CK) concentration at 72 hours post-exercise | At baseline (pre-exercise) and 72 hours post-exercise. | Serum biomarker for muscle damage |
| Change from baseline in creatine kinase (CK) concentration at 96 hours post-exercise | At baseline (pre-exercise) and 96 hours post-exercise.. | Serum biomarker for muscle damage |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Change in maximum voluntary contraction (MVC) from baseline to 96 hours post-exercise | At baseline (pre-exercise) and post-exercise time points (at 0, 24, 48, 72 and 96 hours respectively). | Muscle function measure; maximal voluntary isometric contraction (torque, Nm) performed on isokinetic dynamometer |
| Change in rating of Delayed Onset of Muscle Soreness (DOMS) from baseline to 96 hours post-exercise | At baseline (pre-exercise) and post-exercise time points (at 24, 48, 72 and 96 hours respectively). | Self-reported perception of muscle soreness using a visual analogue scale (VAS), from 'no soreness' \[0 mm\] on the left anchor point to 'extremely sore' \[100 mm\] on the right |
| Change in joint range of motion (ROM) from baseline to 96 hours post-exercise | At baseline (pre-exercise) and post-exercise time points (at 24, 48, 72 and 96 hours respectively). | Elbow ROM measured using a goniometer |
| Change in mid arm circumference (MAC) from baseline to 96 hours post-exercise | At baseline (pre-exercise) and post-exercise time points (at 24, 48, 72 and 96 hours respectively). | Mid-upper arm circumference measured at the midpoint of the distance from the acromion process (acromiale) to the olecranon process (radiale). |
Countries
United Kingdom
Outcome results
None listed