Muscle Loss
Conditions
Brief summary
The primary aim of the current research project is to explore whether leucine stimulates carnitine transport, and thus affecting muscle carnitine content. A secondary aim of this project is to investigate the effect of carnitine with leucine supplementation on muscle strength and body composition.
Interventions
DIETARY_SUPPLEMENTL-carnitine
DIETARY_SUPPLEMENTL-leucine
Sponsors
Medical University of Gdansk
Gdansk University of Physical Education and Sport
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
PREVENTION
Masking
DOUBLE (Subject, Investigator)
Eligibility
Sex/Gender
ALL
Age
18 Years to 29 Years
Healthy volunteers
Yes
Inclusion criteria
* must be able to swallow tablets
Exclusion criteria
* cardiovascular disease * liver disease * kidney disease * neuromuscular disease * gastrointestinal disorders (including stomach ulcers and erosions) * diabetes * other severe chronic diseases
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Muscle Carnitine Concentration using UPLC-MS/MS | 24 weeks | vastus lateralis muscle biopsy taken before and after the supplementation procedure (\ 20 cm proximal to the knee joint using a thin biopsy needle); carnitine concentration determined using ultra performance liquid chromatography - tandem mass spectrometer (UPLC-MS/MS) method |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Muscle Strength using Biodex System 4 Pro dynamometer | 24 weeks | Peak torque measured by performing maximum voluntary contractions during isometric knee extension and flexion. The test performed in concentric-concentric mode consisting maximum 4-s knee extensor isometric contraction and maximum 4-s knee flexor isometric contraction, separated by 20-s recovery, repeated three times. Muscle isokinetic strength assessed in 5 repetitions of flexion and extension at a speed of 60°/s. Muscle endurance assessed in 10 repetitions at 300°/s determine the ability of the muscle to maintain the work. Strength testing performed on the Biodex System 4 Pro dynamometer. |
| Body Composition: Body Fat Mass, Fat Free Mass, and Skeletal Muscle Mass using a bioelectrical impedance analyzer (InBody720). | 24 weeks | The InBody720 measures impedance of five segments of the body (each arm, each leg, trunk) at frequencies of 1, 5, 50, 250, 500, and 1000 kHz through the 8-polar tactile-electrode. Based on the impedance, body fat mass, fat free mass, and skeletal muscle mass are calculated. |
Outcome results
None listed