The Effect of Beetroot Juice on Plasma Trimethylamine N-oxide Concentration

Analysis of Electromyography Activation Trunk Muscles and the Effect of Beetroot Juice Intake on the Results in Strength Exercises: Lateral Medicine Ball Throw and Sled Pushing

Status

UNKNOWN

Phases

Study type

Interventional

Source

ClinicalTrials.gov

Registry ID

NCT05004935

Acronym

BRJ_TMAO

Enrollment

Registered

2021-08-13

Start date

2021-09-01

Completion date

2023-12-15

Last updated

2023-11-15

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Hypertension

Brief summary

The primary aim of the current study is to determine the effect of beetroot juice consumption on plasma trimethylamine N-oxide concentration. A secondary aims: exploring the effect of circulating trimethylamine N-oxide modification on plasma total antioxidant status, and measurement of changes in the skin nicotinamide-adenine dinucleotide reduced form (NADH) content during transient ischemia and reperfusion.

Detailed description

In a random order subjects will consume beetroot juice (140ml), or equimolar nitrates (NaNO3). Antecubital vein blood samples will be taken at the beginning, and every hour for 4 hours following consumption. Plasma trimethylamine N-oxide, total antioxidant status, and nitrite/nitrate concentrations will be determined. Using Flow Mediated Skin Fluorescence skin NADH content determined during controlled ischemia triggered by total occlusion of the brachial artery by the brachial blood pressure cuff and during reperfusion after deflation of the blood pressure cuff 2 and 4 hours following consumption.

Interventions

DIETARY_SUPPLEMENTbeetroot juice

single intake of beetroot juice containing 800mg nitrates

OTHERnitrate

single intake of 800mg nitrates

Study design

Allocation

RANDOMIZED

Intervention model

CROSSOVER

Primary purpose

PREVENTION

Masking

NONE

Eligibility

Sex/Gender

ALL

Age

18 Years to 35 Years

Healthy volunteers

Yes

Inclusion criteria

healthy volunteers

Design outcomes

Primary

Measure	Time frame	Description
The effect of beetroot juice consumption on plasma trimethylamine N-oxide concentration	4 hours	Determination of plasma trimethylamine N-oxide concentration following the beetroot juice consumption using ultra performance liquid chromatography - tandem mass spectrometer (UPLC-MS/MS) method.

Secondary

Measure	Time frame	Description
The effect of circulating trimethylamine N-oxide modification on plasma total antioxidant status	4 hours	Determination of plasma total antioxidant status following the beetroot juice consumption using commercially available kit.

Countries

Poland

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 9, 2026