Bariatric Surgery Candidate, Lipid Metabolism Disorders, Obesity
Conditions
Keywords
Fatty Acids, Nonesterified, Peroxisome Proliferator-Activated Receptors, Endocannabinoids, N-acylethanolamines
Brief summary
The aim of the study is to evaluate modifications in the metabolism of polyunsaturated fatty acids (PUFA) and biosynthesis of the lipid mediators N-acylethanolamines (NAE), both known to induce and be induced by the nuclear peroxisome proliferator-activated receptor (PPAR)-α, which may also explain the well-established improved lipid and energy metabolism after bariatric surgery. The study is focused on understanding the weight loss mechanisms in order to develop targeted dietary protocols.
Detailed description
The study involves a careful preliminary medical history of the patients, with physical examination, anthropometric parameters measurements and blood sampling for blood chemistry routine and evaluation of any adverse events to assess suitability for the study. The evening before bariatric surgery, one month and six months after bariatric surgery anthropometric parameters measurements, blood sampling for blood chemistry routine, inflammatory and lipid-metabolomic profile, and evaluation of any adverse events are performed. For the study samples are stored identified by a code to be analyzed anonymously. Study interruption criteria are: development of allergic reactions, ketonemia\> 1.5 mmol/L, acute gout
Interventions
None listed
Sponsors
Azienda Ospedaliera Brotzu
University of Cagliari
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Healthy volunteers
No
Inclusion criteria
* subjects with pathological obesity suitable for bariatric surgery * Body Mass Index (BMI) range 35-59
Exclusion criteria
* established diagnoses of a major disease (e.g., diabetes or kidney disease) * pregnancy or breastfeeding, * food allergies, * use of drugs that could affect lipid metabolism
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Modifications of Endocannabinoid and N-acylethanolamine plasma levels | 6 months | These lipid derivatives are involved in control of energy expenditure and lipid metabolism and are measured by LC MS/MS and expressed as nmol/ml plasma |
| Weight loss as kg, and fat mass reduction as total kg and percent of body mass | 6 months | Indicators of the success of the intervention |
| Changes in fatty acid plasma profile induced by bariatric surgery | 6 months | Fatty acid profile affect lipid derivatives. Fatty acid are measured by LC and GC and expressed as nmol/ml plasma |
Countries
Italy
Outcome results
None listed