Dysbiosis, Intestinal Disease, Periodontitis
Conditions
Brief summary
Periodontitis is a chronic inflammatory disease of the tooth supporting structures induced by a dysbiosis in the oral and subgingival microenvironment of susceptible patients. The long-term swallowing of high doses of periodontal pathogenic microorganisms could induce a dysbiosis of the intestinal microbiota, favouring the establishment of an 'inflamed' microbiome in terms of composition and/or function. The present project is aimed at a better understanding of the etiopathogenetic correlation between periodontitis and intestinal dysbiosis, and aims to explore the hypothesis that non-surgical periodontal treatment may reduce bacterial alpha diversity in stool samples. Fifty patients affected by stage III-IV periodontitis will be recruited, and treated by means of full-mouth scaling and root planing. Salivary and stool samples, together with a complete periodontal charting and a food diary will be collected and compared at baseline and 3 months after treatment. Age, gender and BMI-matched healthy individuals will be recruited as controls.
Interventions
PROCEDURENon-surgical periodontal treatment
Subgingival instrumentation with ultrasonic devices and curettes of all periodontal pockets
Sponsors
University of Turin, Italy
Study design
Observational model
CASE_ONLY
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to 75 Years
Inclusion criteria
* periodontitis Stage III or IV * BMI between 20 and 29 kg/m2 * free diet * presence of at least 20 teeth
Exclusion criteria
* systemic diseases (including diabetes, thyroid, liver, or kidney diseases) * dietary allergies * use of antibiotics or probiotics during the previous 30 days * pregnancy or breast feeding
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Changes in microbiome alpha diversity measured from stool samples at 3 months | [Time Frame: Baseline and 90 days] | Taxonomic and functional variation of gut bacteria after treatment |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Changes in microbiome alpha diversity measured from saliva samples at 3 months | [Time Frame: Baseline and 90 days] | Taxonomic and functional variation of salivary bacteria after treatment |
| Changes in mean clinical attachment level (CAL) at 3 months | [Time Frame: Baseline and 90 days] | Variation in CAL after therapy assessed using a millimetre marked manual periodontal probe at 6 sites per tooth element |
| Changes in periodontal pocket depth (PPD) at 3 months | [Time Frame: Baseline and 90 days] | Variation in PPD after therapy assessed using a millimetre marked manual periodontal probe at 6 sites per tooth element |
| Changes in full mouth bleeding score (FMBS) at 3 months | [Time Frame: Baseline and 90 days] | Variation in the percentage of bleeding sites after therapy assessed by gentle probing at 6 sites per tooth element |
Countries
Italy
Outcome results
None listed