Healthy
Conditions
Brief summary
The proposed research is a feasibility pilot trial designed to optimize the remote collection dried blood spots (DBS) from a finger stick and the analysis of fatty acid from the DBS and to optimize the analysis of mitochondria activity (or energy metabolism) from peripheral blood mononuclear cells (PBMC) or white blood cell samples. Participants in this study will provide a blood sample from venous blood draw and finger sticks. One finger stick collection will be completed remotely using a DBS kit that is sent through the mail while the other finger stick collection will be completed at the research site. These samples will be use to optimize the method for measuring fatty acids and mitochondria function as well as measure other lipids, insulin, glucose and markers of inflammation using established protocols. Demographic, dietary, sleep, and physical activity information will be measured using questionnaires, while height and weight will be measured to calculate body mass index (BMI).
Detailed description
The primary objectives of the study are * To optimize remote blood collection and laboratory analysis of polyunsaturated fatty acids (PUFAs) in dried blood spot (DBS) samples * To optimize the analysis of mitochondria function in fresh and frozen peripheral blood mononuclear cells (PBMC)
Interventions
None listed
Sponsors
Ohio State University
Study design
Observational model
COHORT
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
* 18 years of age or older * Nonsmoking
Exclusion criteria
* Diabetes
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Optimize mitochondria function measurements in fresh and frozen blood | 1 Day | Measuring oxygen consumption rate in peripheral blood mononuclear cells using seahorse analyzer |
| Optimize remote blood collection and laboratory analysis of in dried blood spot samples | 2 Days | Collecting dried blot spots remotely and in the research setting and venous blood draw in the research setting to compare fatty acid composition |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Assess the relationship of mitochondria function in and cardiolipin in peripheral blood mononuclear cells | 1 Day | Determine mitochondria function in peripheral blood mononuclear cells by measuring oxygen consumption rate and determining its relationship to the mitochondrial phospholipid cardiolipin |
| Assess the relationship of mitochondria function and fatty acid metabolites | 1 Day | Determine mitochondria function in peripheral blood mononuclear cells by measuring oxygen consumption rate and determining its relationship to the fatty acid metabolites in plasma |
| Assess the relationship of mitochondria function and markers of inflammation | 1 Day | Determine mitochondria function in peripheral blood mononuclear cells by measuring oxygen consumption rate and determining its relationship to markers of inflammation in plasma |
| Assess the relationship of mitochondria function in peripheral blood mononuclear cells and markers of glycemia | 1 Day | Determine mitochondria function in peripheral blood mononuclear cells by measuring oxygen consumption rate and determining its relationship to markers of glycemia in serum |
Countries
United States
Outcome results
None listed