Diet, Healthy
Conditions
Brief summary
The investigators will recruit probands with a ileo- or colostoma that are otherwise in good general health and analyse the stomal fluids for their microbiota composition. Sampling will be performed over a time span of 28 days, after 14 days probands will modify their base-line diet to a low carbohydrate diet.
Detailed description
Additionally to stoma fluids which will be analyzed for their microbiota composition the investigators will also collect skin samples to analyze for microbiota composition and urine to analyze for metabolite composition. Low carbohydrate diet will allow for consumption of 80g complex carbohydrates per day, simple sugars will have to be avoided completely
Interventions
OTHERDiet modification
Participants will reduce their carbohydrate consumption to 80g daily
Sponsors
Insel Gruppe AG, University Hospital Bern
Study design
Allocation
NA
Intervention model
SEQUENTIAL
Primary purpose
BASIC_SCIENCE
Masking
NONE
Intervention model description
First phase will be purely observational, second phase involves diet modification
Eligibility
Sex/Gender
ALL
Age
18 Years to 80 Years
Healthy volunteers
Yes
Inclusion criteria
* Ileostomy or colostomy * Good general health * Written informed consent
Exclusion criteria
* Major metabolic diseases that prevent adherence to low carbohydrate diet * Participation in other clinical trial interfering with study protocol
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Assessment of microbiota profile by 16S sequencing before and after diet modification for significant differences | 28 days | Sample content will be measured by 16S sequencing, ecological distances will be assessed by beta-diversity |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Relative changes of total metabolite abundance in urine | 28 days | The whole metabolite composition will be assessed by an untargeted screen done with mass spectrometry |
| Analysis of microbiota metabolic potential (microbiome DNA coding for enzymes) after shotgun sequencing | 28 days | The total DNA content of the samples will be annotated for for known metabolic active enzymes to assess functionality |
| Analysis of microbiota biomass | 28 days | The samples will be analysed for the number of bacteria present in them |
| Changes of further metagenomic microbiome composition (viral, eucaryotic DNA) | 28 days | The DNA of the samples will be screened for parts belonging to viruses or eucaryotic cells |
| Culturing of microbiota if appropriate (in vitro) | 28 days | If of interest, culturing of specific bacterial strains will be attempted in vitro with the aim to recreate in vivo observations |
Countries
Switzerland
Outcome results
None listed