Arterial Stiffness, Dysbiosis
Conditions
Keywords
Lifestyles, pulse wave velocity, intestinal microbiota, multicentric study
Brief summary
This is an observational cross-sectional study whose objective is to analyse associations between the intestinal and salivate microbiota with lifestyles (eating patterns, physical activity, tobacco and alcohol consumption), arterial aging and cognitive function. It will take place in five different research units located in Spain
Detailed description
Aim: To analyze the association of lifestyles (eating patterns, physical activity, tobacco and alcohol consumption) with the intestinal and salivate microbiota and its relationship with arterial aging and cognitive function in subjects without cardiovascular disease in Spain and Portugal. In addition, the mediating role of the intestinal microbiota in the relationship of lifestyles with arterial aging and cognitive function will be analyzed. Design: A cross-sectional study with a case-control analysis, to analyze the association of microbiota patterns with arterial ageing. Setting: the study will develop the research network in health prevention and promotion (Rediapp) and in the Iberian network on arterial structure and the Cancer Research Center of Salamanca. Study population: 1000 subjects aged 45 to 74 years without cardiovascular disease, will be selected. Variables: Demographic, anthropometric and habits (tobacco and alcohol). A dietary pattern through a frequency consumption questionnaire (FFQ), the software of the EVIDENT III and the Mediterranean Diet adherence questionnaire. Physical activity: International Physical Activity Questionnaire (IPAQ), Marshall Questionnaire and Accelerometer, Actigraph. Body composition (Inbody 230 impedance meter). Arterial aging: Medium intimate carotid thickness (Sonosite Micromax); Analysis (PWA) and pulse wave velocity (cf-PWV), (Sphygmocor System). Cardio Ankle Vascular Index (CAVI), ba-PWV and ankle-brachial index (Vasera VS-2000®). With Ambulatory Pulse Wave Analysis (Microsoft®), the PAIx and CAIx and the heart rate variability will be measured. The vascular damage in the retina, heart, kidney, brain and cardiovascular risk will be measured. Intestinal microbiota with the OMNIgene GUT kit (OMR -200) and analysis of the 16S rRNA microbiome profile by massive sequentiation. (Lifestyles, arterial aging, neurocognition, microbiota)
Interventions
None listed
Sponsors
Instituto de Salud Carlos III
Castilla-León Health Service
Instituto de Investigación Biomédica de Salamanca
Study design
Observational model
COHORT
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Age
45 Years to 74 Years
Healthy volunteers
Yes
Inclusion criteria
* People aged 45 to74 years who agree to participate in the study and who not meet any of the
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Pulse Wave Velocity | 1 year | Measurement by SphygmoCor System (meters/seg) |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Cardio Ankle Vascular Index (CAVI) | 1 year | Measurement by Vasera device 2000 (not units) |
| Physical Activity | 1 year | Measurement by the International Physical Activity Questionnaire (IPAQ) (min/day) |
| Eating habits | 1 year | Measurement by Food Frequency Questionnaire (FFQ) (kcal/day) |
| Carotid intima-media thickness (IMT) | 1 year | Measurement by Sonosite Micromax Ultrasound (mm) |
| Drinking habits | 1 year | Measurement by a frequency alcohol questionnaire (units/day) |
| Body composition | 1 year | Measurement by Inbody 230 device (kg) |
| Cognitive evaluation | 1 year | Measurement by Montreal Cognitive Assessment (MOCA) (points). Range: 0-30 points. A score of 26 points or above is considered normal. |
| Smoking | 1 year | Measurement by a questionnaire of four questions adapted from the WHO MONICA study (Smoking status and cigarettes frequency) |
Countries
Spain
Outcome results
None listed