Oral Soft Tissue Conditions, Vasodilatation
Conditions
Keywords
spreading vasodilatation, gingial microcirculation, LSCI
Brief summary
The aim of this study is to compare the endothelim-dependent and non-dependent vasodilatation between genders in the human gingiva.
Detailed description
Gender differences were observed in studies according to gingival microcirculation between physiological circumstances and during wound healing as well. For blood flow measurement Laser Spackle Contrast Imaging is a reliable tool. It has good reproducibility, it doesn't get in touch with the measured surface, it's easy to use and it can measure on a bigger surface in same time. Two wells were fabricated on the labial surface of the right lateral incisor (test tooth) and on the labial surface of the left central incisor (control tooth). These wells are opened towards the high-prermeability gingival sulcus, so the dropped solutions nitroglycerine vs physiological saline or nitroglycerine vs physiological saline can esasily penetrate to the gingival tissues. After applying the solutions, the blood flow was monitored fot 15 minutes.
Interventions
DRUGNitroglycerin
Vasodilatator solution is dropped on the labial surface on the lateral incisor, so it can penetrate through the sulcus.
DRUGAcetylcholine
Vasodilatator solution is dropped on the labial surface on the lateral incisor, so it can penetrate through the sulcus.
Sponsors
Semmelweis University
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
SINGLE (Subject)
Eligibility
Sex/Gender
ALL
Age
20 Years to 30 Years
Healthy volunteers
Yes
Inclusion criteria
* general health * minimum 5 mm keratinized gingiva at the upper front teeth * good oral hygiene
Exclusion criteria
* pregnancy and breast-feeding * smoking * medication * insufficient marginal integrity * periodontitis * caries
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Blood flow change after applying the test solutions | 20 minutes | The change in blood flow will be measeured after applying the test solutions. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Comparison the change in blood flow between the two solutions | 30 minutes | The blood flow values will be evaluated after each applied solutions. |
| Comparison the change in blood flow in genders | 20 minutes | The maximal blood flow changes assessed in primary outcome will be split into two groups based on gender. The change will be compared between males and females. |
Countries
Hungary
Outcome results
None listed