Endothelial Dysfunction, Kidney Diseases
Conditions
Brief summary
Endothelial progenitor cells that reside in renal vasculature may be stimulated to initiate differentiation programs during episodes of injury. It is hypothesized that endothelial progenitor cells resident in the kidney can transition to a post-injury phenotype that promotes endothelial repair.
Detailed description
Endothelial dysfunction is central to the pathophysiology of vascular ischemia, bacterial sepsis, toxin-induced thrombotic microangiopathy, and antibody-mediated kidney transplant rejection and often manifest with renal failure. With each of these diagnoses circulating components of the complement cascade bind to endothelial cells and induce disease progression through anaphylactic cellular messaging, monocyte homing, and direct cell membrane disruption. In response to injury during embryologic development avascular metanephric blastema initiate endothelial differentiation. Although circulating hematopoietic progenitor cells have shown therapeutic promise they incorporate into renal vasculature at very low density.
Interventions
DIAGNOSTIC_TESTCell isolation
Tissue from kidney biopsy will be used to isolate Cluster Differentiation 34+ cells.
Sponsors
Brigham and Women's Hospital
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
BASIC_SCIENCE
Masking
NONE
Intervention model description
Consecutive patients will be consented to obtain kidney biopsy material.
Eligibility
Sex/Gender
ALL
Age
18 Years to 85 Years
Healthy volunteers
No
Inclusion criteria
* Only patients who have a clinical indication for kidney biopsy will be considered. * All subjects will be at least 18 years of age and may be of any gender. * Patients undergoing a native or kidney transplant biopsy will be considered.
Exclusion criteria
* Patients not undergoing a kidney biopsy will be excluded.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Cell growth | 1 week | Tissue will be isolated and cell viability will be documented by rate of colony growth over one week per patient enrolled. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Kidney disease progression | 3 years | Isolated cell growth will be compared to serum creatinine of patients |
Countries
United States
Outcome results
None listed