Muscle Protein Synthesis
Conditions
Brief summary
Dietary protein is vital for the preservation of health and optimal adaptation to training. Plant proteins are considered inferior to animal proteins with respect to their ability to stimulate an acute muscle building response and therefore support long-term muscle reconditioning. Pea protein is a highly commercially available plant proteins source (available as supplements, food ingredients etc.), yet there is no research investigating its ability to stimulate a muscle building response. The investigators aim to assess the effect of consuming pea protein on muscle protein synthesis rates and compare these results to mycoprotein, a source known to elicit a robust anabolic response. Pea protein is lower in some of the essential amino acids, namely methionine, which could mean it is less effective compared with mycoprotein which has a more complete amino acid profile. So in addition to comparing pea with mycoprotein, the investigators also want to compare to a blend of pea and mycoprotein to see if replenishing the amino acid content in pea 'rescues' the anabolic response.
Interventions
DIETARY_SUPPLEMENTBolus ingestion of protein
Ingestion of 25g of protein
Sponsors
Quorn
University of Exeter
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
TRIPLE (Subject, Investigator, Outcomes Assessor)
Eligibility
Sex/Gender
ALL
Age
18 Years to 40 Years
Healthy volunteers
Yes
Inclusion criteria
* BMI between 18 and 30 * Resistance trained
Exclusion criteria
* Any metabolic impairment * Smoking * Use of over the counter pharmaceuticals (excluding oral contraceptives and contraceptive devices). * A personal family history of epilepsy, seizures or schizophrenia. * Allergic to Quorn/mycoprotein/edible fungi/environmental mould products. * Any motor disorder.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Muscle protein synthesis | 8.5 hours | The rate of synthesis of new muscle protein (Fractional synthetic rate %/h) |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Translocation of mTOR via immunohistochemistry | 8.5 hours | Locality of mTOR within the muscle cell |
| mTOR phosphorylation via ATP kinase assay | 8.5 hours | The amount of mTOR that has been activated in the muscle cell |
| Plasma amino acid kinetics | 8.5 hours | The appearance of amino acids from the protein drink in the circulation. |
| Serum insulin concentrations | 8.5 hours | Basal and postprandial concentrations of serum insulin |
Countries
United Kingdom
Outcome results
None listed