Ruminococcus Gnavus, Spondyloarthritis
Conditions
Keywords
Spondyloarthritis, Ruminococcus gnavus, Microbiota, Dysbiosis
Brief summary
The primary objective of this case-control study aims to explore the role of bacterium Ruminococcus gnavus (R. gnavus) with intestinal biopsy and faecal sampling in the initiation and the development of spondyloarthritis (SPA) in comparison with health control subjets (patients without chronic disease but have indication to digestive endoscopy).
Detailed description
As secondary objectives, the study aims: * to research the abundance of bacterial strain of R. gnavus in mucosal sampling by biopsy; * to study the interaction between R. gnavus bacterium and mucus from histological sampling; * to correlate the expression of genes of intestinal mucus with the variations of identified microbiota of mucus biopsy; * to study the interaction between bacteria and intestinal epithelial cells during culture; * to demonstrate perturbations of the immune responses by dysbiosis.
Interventions
PROCEDUREBiopsy
Biopsy during recto-coloscopy
Sponsors
Assistance Publique - Hôpitaux de Paris
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
OTHER
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
Patients with SPA: * Patients ⩾ 18 years; * Diagnosed as spondyloarthritis (SpA) according ASAS classification; * Affiliated to a social security scheme; * Have signed the written informed consent form. Control subjects: * Subjects ⩾ 18 years; * Subjects free of SPA and planned for a digestive endoscopy for another indication such as to explore the risk of colorectal cancer in family member; * Affiliated to a social security scheme; * Have signed the written informed consent form.
Exclusion criteria
* Subjects unable to understand the proposed study and/or sign a informed consent form; * Pregnant women or breast feeding women; * Subjects under guardianship or curatorship; * Have taken antibiotic 1 month prior to inclusion; * Presenting acute or chronic severe pathology may likely to interfere with the interpret of outcome; * Refusal of subjects to participate to the study; * Foreign patients under french AME scheme.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Isolation and characterization of R. gnavus | At the end of study, up to 2 years | Isolation and characterization of R. gnavus bacterium from intestinal biopsy. |
| Bacteria analysis | At the end of study, up to 2 years | Analysis of repartition of R. gnavus bacterium in each group and the comparison between 2 groups. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| RNA sequencing analysis of biopsy | At the end of study, up to 2 years | RNA sequencing analysis of rectal biopsy between SPA patients and control subjects. |
| Bacterial strain's abundance | At the end of study, up to 2 years | Compare the abundance by RT-q-PCR of bacterial strain (R. gnavus) between mucus biopsy sampling and faecal sampling, collected from PCR patients or control subjects respectively. |
| Dysbiosis-induced immune perturbations | At the end of study, up to 2 years | To compare between 2 groups their reactivities of T cells from intestinal biopsy which contact with cells of exposed antigens in vitro, to R. gnavus stains or to other stains biopsied from control subjects. |
| Interaction between bacteria and intestinal epithelial cells | At the end of study, up to 2 years | In order to study the interaction between bacteria and intestinal epithelial cells in culture (organoids). Study the induction of several target genes as peptides anti-microbians, mucin or chemokines by RT-q-PCR. |
| Mucin-degradation of Ruminococcus gnavus | At the end of study, up to 2 years | Study the ability of the strain to grow on mucins of intestinal biopsy, colored after fixation to carnoy. |
Countries
France
Contacts
Primary ContactMaxime BREBAN, MD, PhD
Outcome results
None listed