Gingivitis, Healthy, Periodontitis
Conditions
Keywords
NLRC4
Brief summary
NLRC4, an inflammasome , is a cytosolic multiprotein complex involved in the initiation and modulation of the immune response. Periodontitis is a chronic inflammatory disease that can start with localized inflammatory reactions created by the supporting tissues surrounding the teeth against microorganisms and then result in loss of teeth. It has been said that proinflammatory cytokines released in the microenvironment of periodontitis can increase the expression of NLRC4 inflammasome genes. The authors think that NLRC4 may play a role in the periodontitis. The aim of this study is to compare the NLRC4, IL-1β and IL-10 levels of healthy and periodontitis individuals.
Detailed description
Periodontitis is a destructive chronic inflammatory disease that can start with localized inflammatory reactions created by the supporting tissues surrounding the teeth against microorganisms and then result in loss of teeth. Inflammasomes are cytosolic multiprotein complex involved in the initiation and modulation of the immune response. Interleukine -1β ( IL-1β ) levels are associated with periodontal destruction in the pathogenesis of periodontal disease. Interleukine-10 (IL-10) has been reported to decrease periodontal disease progression rate. Gingival crevicular fluid shows the cellular response in the periodontium. The most important host-related ingredients are inflammatory markers, including cytokines, enzymes, and interleukins. Saliva analysis is used in the diagnosis of systemic conditions as well as in the diagnosis of oral pathologies. Both NLRC4 and NLRP3 inflammasomes have been shown to play an important role in the induction of interleukin-1β secretion. Periodontitis is induced by a complex microbial biofilm containing multiple Gram-positive and Gram-negative bacteria (including flagellate microorganisms) capable of activating a large number of NLRs. It has been said that proinflammatory cytokines released in the microenvironment of periodontitis can increase the expression of NLRC4 inflammasome genes. Evidence on the role of inflammasomes in periodontal disease is still very poor. The aim of this study is to compare the NLRC4, IL-1β and IL-10 levels of healthy and periodontitis individuals.
Interventions
OTHERSaliva
individuals' saliva samples will be collected and transferred to eppendorf tubes. Until biochemical analysis is carried out will be stored in -80ºC.
GCF samples of individuals will be collected by paper strips and transferred to eppendorf tubes.Until biochemical analysis is carried out will be stored in -80ºC.
Sponsors
Saglik Bilimleri Universitesi
Study design
Observational model
OTHER
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Age
18 Years to 70 Years
Healthy volunteers
Yes
Inclusion criteria
* systemically healthy individuals who does not have a disease under treatment, individuals without regular systemic medication
Exclusion criteria
* smoking receiving periodontal treatment in the last 6 months pregnancy menopausal or menstrual period
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| IL-1β levels | through study completion, an average of 6 months | saliva and DOS levels |
| IL-10 levels | through study completion, an average of 6 months | saliva and DOS levels |
| NLRC4 | through study completion, an average of 6 months | saliva and DOS levels |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Plaque Index | up to 20 weeks | Silness-Löe |
| Probing Depth | up to 20 weeks | millimeter |
| Bleeding on Probe | up to 20 weeks | Ainamo&Bay |
Countries
Turkey (Türkiye)
Contacts
Primary ContactAyşe Toraman, Dr
Outcome results
None listed