Inflammatory Response
Conditions
Brief summary
The aim of the present study is to determine the effect of Xanthohumol and Iso-alpha-acids from hops on the immune response of healthy participants.
Detailed description
In this placebo-controlled crossover study a study drink enriched with either a placebo, Xanthohumol, Iso-alpha Acids or a combination of latter is given to the participants. A washout phase before intervention and between each intervention is introduced. On day one of each intervention blood is drawn in fasted state from each participant. Subsequently the study drink combined with a standardized breakfast is given to the participants following a second blood drawing one hour after the consumption of the study drink. On the last day of each intervention blood is taken from study participants in the same manner as on day one. To assess the effect of Xanthohumol and Iso-alpha acids or a combination of both, clinical chemical parameters e.g. ALT, AST, γ-GT, blood lipids (triglycerides, total cholesterol, HDL, LDL), blood glucose, uric acid and CRP are determined. Furthermore, PBMC's isolated from blood samples of each time point were stimulated for either 24 or 48 h. Protein levels and mRNA expression of markers of inflammation is measured in PBMC's and supernatant of stimulated cells.
Interventions
OTHERDietary Intervention 1: Placebo
Participants receive a study drink supplemented with a placebo for 5 days
OTHERDietary Intervention 2: Xanthohumol and Iso-alpha acids
Participants receive a study drink supplemented with xanthohumol, Iso-alpha acids or a combination of both for 5 days
Sponsors
University of Vienna
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
SINGLE (Subject)
Eligibility
Sex/Gender
ALL
Age
18 Years to 30 Years
Healthy volunteers
Yes
Inclusion criteria
* healthy, normal weight,
Exclusion criteria
* food intolerances, food allergies, chronic inflammatory diseases, metabolic diseases, viral or bacterial infections within the last 3 weeks of inclusion, intake of immunosupressive medication
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Changes in parameters of immune response | 5 days | Parameters of immune response are measured in cell culture supernatant of stimulated cells |
Countries
Austria
Outcome results
None listed