Critical Illness With or Without Sepsis
Conditions
Brief summary
Vascular dysfunction is an important mechanism involved in organ failure, in the setting of sepsis condition, with different types of circulating endothelial cells.Transcriptom analysis via RNAseq in different types of circulating endothelial cells, comapring critically ill patients with or without sepsis will allow determining differential gene expression for signal pathways in endothelial alteration and restoration associated with sepsis.
Interventions
OTHERObservational study
Observational study. Groups are defined based on baseline patients' characteristics : presence or absence of sepsis.
Sponsors
Assistance Publique - Hôpitaux de Paris
Study design
Observational model
OTHER
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to 85 Years
Healthy volunteers
No
Inclusion criteria
* Age between 18 and 85 years * Admission in intensive care with or without sepsis * Health insurance as per the national insurance system * Informed and unopposed patient for participation (or referend closed one)
Exclusion criteria
* Patient under legal protection * Patient under national medical help system * Pregnancy
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Gene expression based on transcriptom analysis of circulating endothelial cells | Day 1 | Gene expression based on transcriptom analysis of circulating endothelial cells (RNAseq) |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Sequential Organ Failure Assessment (SOFA) score | Day 1, Day 3, Day 7 | Sequential Organ Failure Assessment (SOFA) score, ranging from 0 (best: absence of organ failure) to 24 (worse: multiple organ failure) |
| Sequential Organ Failure Assessment (SOFA) hemodynamic sub-score | Day 1, Day 3, Day 7 | Sequential Organ Failure Assessment (SOFA) hemodynamic sub-score, ranging from 0 (best: absence of hemodynamic failure) to 4 (worse: hemodynamic failure) |
| Sequential Organ Failure Assessment (SOFA) respiratory sub-score | Day 1, Day 3, Day 7 | Sequential Organ Failure Assessment (SOFA) respiratory sub-score, ranging from 0 (best: absence of respiratory failure) to 4 (worse: respiratory failure) |
| Sequential Organ Failure Assessment (SOFA) neurological sub-score | Day 1, Day 3, Day 7 | Sequential Organ Failure Assessment (SOFA) neurological sub-score, ranging from 0 (best: absence of neurological failure) to 4 (worse: neurological failure) |
| Sequential Organ Failure Assessment (SOFA) hematological sub-score | Day 1, Day 3, Day 7 | Sequential Organ Failure Assessment (SOFA) hematological sub-score, ranging from 0 (best: absence of hematological failure) to 4 (worse: hematological failure) |
| Intensive care unit (ICU) mortality | ICU length of stay | Death during ICU stay |
| D28 mortality | Day 28 | Death within 28 days of intensive care unit |
| Hospital mortality | Day 90 | Death within 90 days of intensive care unit |
| Duration of ventilation | Day 28 | Number of days alive without ventilation up to Day 28 |
| Counts of circulating endothelial cells | Day 1 and Day 3 | Counts of circulating endothelial cells, including progenitor cells |
| Duration of vasopressors therapy | Day 28 | Number of days alive without vasopressors up to Day 28 |
| Dose of vasopressors | ICU length of stay, assessed up to 90 days | Dose of vasopressors, in patients who received vasopressors |
| Duration of intensive care unit (ICU) stay | Day 28 | Number of days alive outside the ICU up to D28 |
| Circulating endothelial cells cytokines profile | Day 1 and 3 | Dosage of cytokines from circulating endothelial cells |
| Circulating endothelial cells chemokines profile | Day 1 and 3 | Dosage of chemokines from circulating endothelial cells |
| Expression of membrane proteins of endothelial cells | Day 1 and 3 | Expression of membrane proteins of endothelial cells by flow cytometry |
| Infection site | Day 1 | Infection site for patients with sepsis |
| Infection identification | Day 1 | Identified germs for patients with sepsis |
| Number of participants with different types of antibiotic treatment | Length of ICU stay, assessed up to 90 days | Antibiotic treatments for patients with sepsis during intensive care unit (ICU) stay |
| Duration of renal replacement therapy (RRT) | Day 28 | Number of days alive without RRT up to Day 28 |
Countries
France
Contacts
Primary ContactLara Zafrani, MD PhD
Outcome results
None listed