Glioma
Conditions
Keywords
pathologist-graded gliomas
Brief summary
The investigators showed in 2015 (Guichet et al) that the Notch1 pathway has an anti-proliferative role on glial brain tumors. In this project, the investigators investigated which genes downstream of Notch1 pathway activation produce the anti-proliferative effect. The investigators hypothesized that the SNAI2 and TAL1 genes act downstream of Notch1
Detailed description
Main objectives: To examine the expression of TAL1 and SNAI2 in protein extracts and glioma sections of different grades (glioblastomas and diffuse low-grade tumors). To examine the effect of overexpression of Tal1 and Snai2 in high grade glioma cultures. To show the expression of Tal1 and SNAI2 by tumor cells by FISH technique. Show that TAL1 and SNAI2 could be regulated by endothelial cells
Interventions
None listed
Sponsors
University Hospital, Montpellier
Study design
Observational model
CASE_ONLY
Time perspective
RETROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* Patient ≥18 years old * Patient affected with brain glioma classified according to WHO 2016 classification
Exclusion criteria
* Patient who reject the study protocol * Radiotheray or chemotherapy treatment
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Effect of Notch1 activation on Tal1 | 1 day | highthroughput gene analysis (DNA arrays) with cells transduced with activated form of Notch1 |
| Snai2 gene and protein expression | 1 day | western blot for Tal1 and Snai2 in protein extracted from low grade and high gliomas |
Countries
France
Outcome results
None listed