Vitreoretinal Detachment, Vitreo-Retinal Adhesion
Conditions
Keywords
internal limiting membrane, human vitreous, vitreomacular adhesion, vitreoretinal contact, vitreoretinal interface, hyalocytes, collagen
Brief summary
Investigation of the vitreoretinal contact microarchitecture in the macular and paramacular zones of the human retina.
Detailed description
Purpose: to study the features of the macular ILM, to study the features of the preretinal structures of the vitreous body of the Central retinal region, morphofunctional features of preretinal hyalocytes, and the mechanism of vitreoretinal adhesion. Study Protocol: examination of donor human eyes obtained from an eye Bank after sampling material for corneal transplantation. Research method: scanning electron microscopy (SEM) of the central retina (macular and paramacular zone): ILM, preretinal vitreous. Scientific hypothesis: vitreoretinal adhesion is provided not by laminin and fibronectin, but by the interweaving of vitreous collagen fibers between the epiretinal vitreous (more firmly fixed to the retinal surface) and the cortical vitreous. Expected results: determination of ILM thickness in the macular zone, microanatomy of preretinal vitreous strutcures, and possible mechanism of vitreoretinal adhesion, establishing the possibility of intravital synthesis of collagen fibers synthesis in the adult vitreous.
Interventions
scanning electron microscopy of the сentral retina and preretinal structures
Sponsors
The S.N. Fyodorov Eye Microsurgery State Institution
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
35 Years to 45 Years
Healthy volunteers
No
Inclusion criteria
* eyes from donors without concomitant multiple organ pathology and ophthalmic pathology
Exclusion criteria
* eye injury, age over 45 years, concomitant multiple organ pathology, previous eye diseases
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| the ILM thickness in the macular zone (foveola) | within 3 days after receiving the cadaver eyes | the ILM thickness in the macular zone (foveola) in mkm by scanning electron microscopy data |
| superficial microstructure of ILM | within 3 days after receiving the cadaver eyes | by scanning electron microscopy data |
| features of the structural microarchitecture of the preretinal vitreous in the macular zone | within 3 days after receiving the cadaver eyes | by scanning electron microscopy data |
| morphofunctional features of preretinal hyalocytes. | within 3 days after receiving the cadaver eyes | by scanning electron microscopy data |
Countries
Russia
Outcome results
None listed