Physical Activity
Conditions
Keywords
Isometric contraction exercise
Brief summary
The goal of the study is to determine whether aerobic or resistant exercise can improve in vivo mitochondrial capacity of skeletal muscle cells similarly in healthy younger, middle aged and older adults. This confers long-term changes in this tissue which in-turn contribute to improved metabolic health and functional capacity through epigenetic regulation of novel exercise response genes.
Detailed description
The goal of the study is to understand the biological pathways and molecules driving the exercise response variation of the muscle mitochondria in vivo to elucidate the underlying mechanisms for the health benefits of exercise training in young, middle aged and older adults. This knowledge will improve investigators understanding of biological basis for targeted precision medicine exercise and non-exercise interventions to improve age related conditions. The mitochondrial capacity measurement via non-invasive 31P magnetic resonance spectroscopy (31-MRS) will provide a tangible link between the two primary foci of the parent MoTrPAC study: molecular drivers of exercise response and key phenotypic health outcomes.
Interventions
OTHERIsometric Contraction Exercise - Young Adults (Age group 18-39)
Subjects will be asked to lie supine on MRI table with the 31P surface coil placed over the right vastus lateralis, and straps to immobilize the leg. At the start of the scan session, the investigators will instruct the participant on the isometric contraction exercise and coach the participant through a demonstration. The average flexion repetition time is on the order of 1.5 s for a 30 s duration at maximum kick intensity.
OTHERIsometric Contraction Exercise - Middle Age Adults (Age group 40-59)
Subjects will be asked to lie supine on MRI table with the 31P surface coil placed over the right vastus lateralis, and straps to immobilize the leg. At the start of the scan session, the investigators will instruct the participant on the isometric contraction exercise and coach the participant through a demonstration. The average flexion repetition time is on the order of 1.5 s for a 30 s duration at maximum kick intensity.
OTHERIsometric Contraction Exercise - Old Adults (Age group >60)
Subjects will be asked to lie supine on MRI table with the 31P surface coil placed over the right vastus lateralis, and straps to immobilize the leg. At the start of the scan session, the investigators will instruct the participant on the isometric contraction exercise and coach the participant through a demonstration. The average flexion repetition time is on the order of 1.5 s for a 30 s duration at maximum kick intensity.
Sponsors
University of Colorado, Denver
University of Pittsburgh
The University of Texas Health Science Center at San Antonio
AdventHealth Translational Research Institute
Stanford University
Wake Forest University Health Sciences
Pennington Biomedical Research Center
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
OTHER
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
ADULT PARTICIPANT INCLUSION CRITERIA - YOUNG PARTICIPANTS * Willingness to provide informed consent to participate in the MoTrMito Study * Must be able to read and speak English well enough to provide informed consent and understand instructions * Aged 18 - 39 y * Body Mass Index (BMI) \>19 to \<35 kg/m2 ADULT PARTICIPANT INCLUSION CRITERIA - MIDDLE AGE PARTICIPANTS * Willingness to provide informed consent to participate in the MoTrMito Study * Must be able to read and speak English well enough to provide informed consent and understand instructions * Aged 40 - 59 y * Body Mass Index (BMI) \>19 to \<35 kg/m2 ADULT PARTICIPANT INCLUSION CRITERIA - OLD AGE PARTICIPANTS * Willingness to provide informed consent to participate in the MoTrMito Study * Must be able to read and speak English well enough to provide informed consent and understand instructions * Aged \>=60 y * Body Mass Index (BMI) \>19 to \<35 kg/m2
Exclusion criteria
ADULT PARTICIPANT
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Pre-training 31P MRS measurement - Phosphocreatine recovery rate | Baseline visit - before any muscle biopsies or exercise training. | The plot of PCr peak height vs. time will be fitted with a mono-exponential equation whose free parameters are the recovery time constant (tau) and scaling coefficients. Percent change in tau between pre- and post-intervention scans will be our primary measurement of the maximal mitochondrial capacity response to exercise training. |
| Post-training 31P MRS measurement - Phosphocreatine recovery rate | After week 12 visit - after all muscle biopsies and exercise training. | The plot of PCr peak height vs. time will be fitted with a mono-exponential equation whose free parameters are the recovery time constant (tau) and scaling coefficients. Percent change in tau between pre- and post-intervention scans will be our primary measurement of the maximal mitochondrial capacity response to exercise training. |
Countries
United States
Outcome results
None listed