Asthma, Covid19
Conditions
Brief summary
To assess if TSLP expression by airway epithelial cells following COVID-19 infection regulates viral load and disease severity.
Detailed description
The study aim is to determine the relationship between COVID-19 infection and epithelial cell expression of TSLP. Two cohorts will be used. Cohort One will include 100 hospitalized patients with Covid-19, and 100 hospitalized patients without Covid-19. Cohort 2 will include mild/moderate asthmatics and healthy controls with bio-banked biopsy material used from donors who have previously consented for samples to be used for future research.
Interventions
None listed
Sponsors
McMaster University
Study design
Observational model
COHORT
Time perspective
RETROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
* Age 18 years and above; * Able to give informed consent; * Clinically tested for SARS-CoV-2 (as completed by standard laboratory testing e.g. RT-PCR) and with a positive result and clinical features in keeping with COVID-19 illness. The control group will be patients with a negative RT-PCR COVID-19 test and without any clinical features to suggest COVID-19 illness. In addition, the control group should have NO known or current features of respiratory infection, COPD, asthma, interstitial lung disease, other respiratory inflammatory conditions or lung cancer. * Able to provide a breath sample (as based on clinical judgement).
Exclusion criteria
\- none
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| TSLP expression in nasal epithelium | Through study completion, an average of 1 year | Compare the level of TSLP expression in nasal epithelium in patients with and without COVID-19 infection |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| TSLP expression in endobronchial biopsies | Baseline | Epithelial cell expression (protein staining) of TSLP in endobronchial biopsy tissue of asthmatic patients versus normal controls. |
| IL-33 expression in endobronchial biopsies | Baseline | Epithelial cell expression (protein staining) of IL-33 in endobronchial biopsy tissue of asthmatic patients versus normal controls. |
| ACE-2 receptor expression in endobronchial biopsies | Baseline | Epithelial cell expression (protein staining) of ACE-2 receptor in endobronchial biopsy tissue of asthmatic patients versus normal controls. |
Countries
Canada
Contacts
Primary ContactGail Gauvreau, PhD
Outcome results
None listed