Covid19
Conditions
Brief summary
The aim of this study is to confirm or rule out the residual presence and viability of SARS- CoV-2 in the respiratory tract and lung parenchyma of patients previously infected with SARS-CoV-2. Presence and viability of SARS-CoV-2 in lung tissue will be assessed with reverse transcriptase-polymerase chain reaction (PCR) and viral culture. The histological location of residual SARS-CoV-2 will be determined with fluorescence immunohistochemistry and single molecule fluorescence in situ hybridization, targeting viral proteins and RNA respectively.
Detailed description
The investigators will collect residuary lung tissue after medically indicated pulmonary resections at the Thoracic Surgery Department of University Hospitals Leuven. More specifically the investigators will collect lung tissue from patients that had earlier PCR proven COVID-19 and/or serum anti-SARS-CoV-2 antibodies. Furthermore, for negative control of the methodology the investigators will also collect lung tissue from patients that have no signs for earlier SARS-CoV-2 infection.
Interventions
DIAGNOSTIC_TESTLung biopsy
Biopsy of residuary material from a medically indicated pulmonary resection
Sponsors
Universitaire Ziekenhuizen KU Leuven
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Inclusion criteria
* Patient requires medically indicated pulmonary resection * Informed consent
Exclusion criteria
* Active SARS-CoV-2 infection/COVID-19 * No informed consent
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Reverse transcriptase-PCR | Single time point. | Detection of SARS-CoV-2 RNA in lung tissue homogenate with primers targeting both genomic and subgenomic RNA |
| Fluorescence immunohistochemistry (IHC) | Single time point. | Determining the histological presence of SARS-CoV-2 viral proteins in lung tissue with commercially available antibodies against the SARS-CoV-2 spike and nucleocapsid proteins |
| Single molecule fluorescence in situ hybridization (smFISH) | Single time point. | Determining the presence of SARS-COV-2 viral RNA in lung tissue with commercially available probes targeting both sense and anti-sense viral RNA |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Viral culture | Single time point. | Determining viability of SARS-CoV-2 in case of a low revere transcriptase-PCR cycling threshold |
Countries
Belgium
Contacts
Primary ContactLaurens J Ceulemans, MD, PhD
Outcome results
None listed