Periodontitis
Conditions
Brief summary
This study aimed to assess clinically and biochemically the effect of intrapocket application of tea tree oil (TTO) gel adjunctive to SRP in the management of stage 2(moderate) periodontitis and to correlate the biochemical levels with clinical response.
Detailed description
A randomized, controlled clinical trial included thirty patients with stage 2 periodontitis. They were equally divided into two groups: Group 1 (control group) treated with scaling and root planning (SRP) alone and Group 2 (test group) managed by SRP and locally delivered 5% tea tree oil (TTO) gel.
Interventions
PROCEDUREScaling and Root Planing
Supra- and sub-gingival calculus and debris were removed
DRUGScaling and Root Planing with tea tree oil
Supra- and sub-gingival calculus and debris were removed and 5% tea tree oil was applied
Sponsors
Alexandria University
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
TREATMENT
Masking
SINGLE (Outcomes Assessor)
Eligibility
Sex/Gender
ALL
Age
20 Years to 40 Years
Healthy volunteers
No
Inclusion criteria
* Patients diagnosed with stage 2,grade B periodontitis according to the 2017 World Workshop on the Classification of Periodontal and Peri-Implant Diseases and Conditions. * Patients with CAL 3-4 mm , BOP and radiographic horizontal bone loss related to the coronal third of the root (15%-33%). * Patients with no teeth loss due to periodontitis. * Patients with radiographic bone loss/age % of 0.25-1 %. * Patients who could maintain an O'Leary plaque index ≤10 proceeded into the study.
Exclusion criteria
* Patients with CAL caused by non periodontal causes. * Patients with grade C periodontitis. * Patients having any systemic disease that may affect the treatment outcomes * Smokers. * Pregnant females. * Patients receiving contraindicated medications, chemotherapy, or radiotherapy in the previous year.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Probing depth | up to 6 months | Probing depth was recorded at tested sites with graduated William's probe graduated in 1 mm increment. Readings were rounded to the nearest mm. |
| Clinical attachment loss | up to 6 months | Clinical attachment loss was recorded at tested sites with graduated William's probe graduated in 1 mm increment. Readings were rounded to the nearest mm. |
| Bleeding on probing | up to 6 months | Bleeding on probing was assessed at tested sites with graduated William's probe graduated in 1 mm increment, within 15 seconds after probing, using a dichotomous scoring system (+ and -) for presence or absence, respectively. |
| Biochemical assessment of inlammation | up to 6 months | This involved determination of the gingival crevicular fluid (GCF) levels of matrix metalloproteinase 8 (MPP-8). A GCF sample was taken from the area showing the deepest pocket depth around the treated area.The samples were diluted in phosphate buffer saline (PBS) up to 1 mL. After waiting for 15 min, the paper points were removed, and 500 μL were centrifuged at 400 g for 4 min, then frozen at -20°C pending MMP-8 analysis using Human Matrix metalloproteinase 8/Neutrophil collagenase (MMP-8) ELISA Kit according to the manufacturer instructions. |
Countries
Egypt
Outcome results
None listed