Sepsis
Conditions
Keywords
sTREM-1, sUPAR, CRP
Brief summary
This study aims to evaluate sepsis biomarkers as soluble triggering receptor expressed on myeloid cells 1 (sTREM-1) and soluble urokinase plasminogen activator receptor (sUPAR) in diagnosis of sepsis in comparison to the traditional blood culture and C-reactive protein (CRP) and to evaluate the prognostic value of these biomarkers in comparison to sequential organ failure assessment score (SOFA score), Acute Physiology and Chronic Health Evaluation II ( APACHI score), 28 day mortality.
Detailed description
Sepsis is usually diagnosed in patients have clinically suspected infections with systemic inflammatory response syndrome (SIRS) manifestations. Patients with SIRS as those with trauma or stroke have the same classic infection signs such as fever and elevated leukocytic count, as long as the microbiological diagnosis is the ultimate diagnostic method, but it has poor sensitivity with delayed results. New biomarkers such as sTREM-1 and sUPAR can be used for rapid diagnosis of sepsis and differentiating it from non infectious inflammatory syndromes. Also these soluble biomarkers can be used for predicting the prognosis of sepsis patients
Interventions
None listed
Sponsors
Sohag University
Study design
Observational model
OTHER
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Age
19 Years to 70 Years
Inclusion criteria
* Temperature of \>38oC * Heart rate of \>90 beats/min * Respiratory rate of \>20 breaths/min * Partial pressure of arterial carbon dioxide (PaCO2) of \<32 mmHg * White blood cell (WBC) count of \>12,000 cells/mm3
Exclusion criteria
* HIV patients * Patients with neutropenia \<1000 cells/mm3 * \<18 years of age.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Measurement of the serum level of sTREM-1 (ng/L) in the patients with criteria of SIRS | in the 1st day after appearance of clinical signs of infection | by ELISAenzyme linked immunosorbent assay |
| Measurement of the serum level of sUPAR (ng/L) in the ICU patients | in the 1st day after appearance of clinical signs of infection | by ELISAenzyme linked immunosorbent assay |
| Measurement of the serum level of sUPAR (ng/L) in the patients with criteria of SIRS | in the 7th day after appearance of clinical signs of infection | by ELISAenzyme linked immunosorbent assay |
| Measurement of the serum level of CRP (mg/dl) in the patients with criteria of SIRS | in the 1st day after appearance of clinical signs of infection | by turbidimetry |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Acute Physiology and Chronic Health Evaluation II (APACHE II) score designed to measure the severity of disease for adult patients admitted to intensive care units in the with criteria of SIRS | in the 7th day after appearance of clinical signs of infection | an integer score from 0 to 71 |
| The sequential organ failure assessment score (SOFA score) had been used to determine the extent of organ dysfunction of ICU patients with criteria of SIRS | in the 7th day after appearance of clinical signs of infection | 0-\<11 |
Countries
Egypt
Outcome results
None listed