HCC
Conditions
Brief summary
Netrin-1 is a dependence receptor ligand participating in the pathology of several cancer types. It is up-regulated in chronic liver diseases, cirrhosis and HCC. We hypothesize that netrin-1 may play a detrimental role in HCC. The goal of this project is to characterize netrin-1 signals in HCC samples with ad hoc controls, to investigate the benefit of capturing netrin-1 in preclinical models of HCC and to try to define patients groups the most likely to benefit from this targeting approach in the clinic.
Interventions
PROCEDUREQuantification of netrin-1 signals by antibody-based approaches
Samples have been obtained upon curative surgery without any other therapy administered and subsequently characterized by pathologists after FFPE inclusion.
Sponsors
Hospices Civils de Lyon
Study design
Observational model
COHORT
Time perspective
RETROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to 85 Years
Healthy volunteers
No
Inclusion criteria
* any patient suffering from HCC * age \>18 * informed consent provided
Exclusion criteria
\- absence of informed consent
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Netrin-1 expression patterns in HCC samples | Outcome measure is to be performed after surgery of the tumors (at least 1 year later). It has no impact on the patient's care. | Netrin-1 expression levels will be quantified by immunoblotting with ad hoc internal standards. Positivity threshold by IHC will be evaluated using ad hoc positive and negative control tissues. The densitometry approach will be used. |
Countries
France
Contacts
Primary ContactFabien ZOULIM, Pr
Backup ContactRomain PARENT
Outcome results
None listed