Glycocalyx, Liver Transplantation
Conditions
Brief summary
An organ intended for transplantation is normally stored on ice (cold storage, CS) after explantation from the donor. During this storage process, damage to the endothelial glycocalyx occurs. It is known from numerous studies that the integrity of the endothelial glycocalyx is necessary for organ function. Normothermic machine perfusion is an alternative storage method for explanted livers, where the graft is perfused with warm blood. This study aims to clarify the influence of normothermic machine perfusion on endothelial glycocalyx damage of the graft.
Interventions
DIAGNOSTIC_TESTHeparansulfate and Syndecan 1
Blood samples will be collected from the patient at 5 different time points. Three perfusate samples will be collected during normothermic machine perfusion (4,9ml each).
Sponsors
Medical University Innsbruck
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* First deceased donor liver transplantation receiving a graft after normothermic machine perfusion * Recipient age \>18 years * Written informed consent
Exclusion criteria
* Age \< 18 years
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Syndecan 1 level during normothermic machine perfusion | 24 hours | Syndecan 1 level at the end of normothermic perfusion compared to the beginning of perfusion |
| Heparansulfate level during normothermic machine perfusion | 24 hours | Heparansulfate level at the end of normothermic perfusion compared to the beginning of perfusion |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Syndecan 1 after transplantation | 72 hours | Change of Syndecan 1 level after transplantation |
| Heparansulfate level after transplantation | 72 hours | Change of Syndecan 1 level after transplantation |
Countries
Austria
Outcome results
None listed