Basal Cell Carcinoma
Conditions
Keywords
Basal Cell Carcinoma, Squamose Cell Carcinoma, Actinic Keratoses, SMAD4 methylation, SMAD4 expression
Brief summary
Non-melanocytic skin cancers are the most common type of cancer worldwide. In the development of this cancer type, environmental factors such as UV and smoking are emphasized. Epigenetics are genetic conditions that develop due to environmental factors and can be inherited. Epigenetic modifications such as DNA methylation play an integral role in carcinogenesis, cancer progression and metastasis. The TGF-/ SMAD4 pathway plays a tumor suppressive role in cancer pathogenesis. Epigenetic changes in this pathway also lead to a decrease in expression level, leading to different types of cancer. However, there is no study showing the epigenetic relationship between non-melanocytic skin cancer and SMAD4 methylation. In this study we planned, it was aimed to show the change in SMAD4 methylation and SMAD4 RNA expression level in cancerous tissue. In addition, it is planned to measure the SMAD4 protein positivity rate in non-melanocytic cancers as an immunohistochemical marker. In this context, 60 patients who applied to Trakya University Dermatology and Venereal Diseases Outpatient Clinic and diagnosed with non-melanocytic skin cancer clinically and dermoscopically will be included in the study. Tissue materials obtained from both cancerous and intact skin of the patients will be examined in Trakya University Medical Biophysics and Medicine Pathology laboratories through various steps. Our project is the first study to be conducted on this subject in terms of handling all non-melanocytic skin cancers, using human tissue and having a large sample. In addition, with the data to be obtained; We think that better clarification of the role of SMAD4 in non-melanocytic cancers and the use of SMAD4 as both a prognostic factor and an immunohistochemical marker in future studies will prevent this study. Again, we anticipate that different treatment modalities will be developed and different functional studies can be designed through this pathway.
Interventions
OTHERSMAD4 expression and methylation
Effect of SMAD4 methylations on smad4 expression
Sponsors
Trakya University
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
BASIC_SCIENCE
Masking
NONE
Intervention model description
Differantiation of tumor tissues than normal tissues in same patients.
Eligibility
Sex/Gender
ALL
Age
18 Years to 80 Years
Healthy volunteers
No
Inclusion criteria
1. People diagnosed clinically and dermoscopically with non-melanocytic skin cancer 2. No other cancer history 3. over 18 years old 4. Newly diagnosed 5. No systemic or topical therapy
Exclusion criteria
1. Under 18 and pregnant 2. People with non-melanocytic skin cancer who are in the process of follow-up and treatment 3. has another cancer history
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Detection of SMAD4 methylations and expression | 2 years | Detection of SMAD4 methylations on tumor tissues |
Countries
Turkey (Türkiye)
Contacts
Primary ContactYildiz Gursel, MD
Outcome results
None listed