Infertility, Female
Conditions
Brief summary
The purpose of this study is to identify a systemic immunological profile specific to patients with recurrent implantation failures (RIF) or miscarriages (RM) through blood immunophenotyping on the day of ovulation. This study also aims to determine whether the intrauterine administration of 5 million peripheral blood monocluear cells increases implantation and pregnancy rates in patients with RIF and decreases the risk of miscarriages in patients with RM, compared to what has already been published in the scientific literature
Interventions
Intrauterine administration of hCG-activated peripheral blood mononuclear cells
Sponsors
Centre hospitalier de l'Université de Montréal (CHUM)
Fertilys
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
DIAGNOSTIC
Masking
NONE
Eligibility
Sex/Gender
FEMALE
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* All women aged 18 and over undergoing an IVF cycle with her partner's sperm at the Fertilys fertility center, without a RIF status, agreeing to participate in the study and having signed the consent form to participate in the study. * All women aged 18 and over undergoing an IVF cycle with her partner's sperm at the Fertilys fertility center, with a RIF or RM status, agreeing to participate in the study and having signed the consent form to participate in the study.
Exclusion criteria
* Women who need a gametes donor (eggs and / or sperm). * Women undergoing an IVF cycle with frozen sperm. * Women with autoimmune disease. * Women with diabetes. * Women with a history of chemotherapy treatment.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Embryo implantation rate (biochemical pregnancy) | Two weeks following embryo transfer or 3 weeks following intrauterine insemination |
| Clinical pregnancy rate | Six to 8 weeks of gestation |
Secondary
| Measure | Time frame |
|---|---|
| Peripheral blood mononuclear cells populations (PBMC) distribution at Day 0 | The day 0 of the patient's ovulation |
| Peripheral blood mononuclear cells populations distribution at Day 2 | After 48 hours of culture |
| Cytokines (LIF, IL-6, IL-10, TNFα, FGF-2, VEGF, IL-8) quantification by ELISA in PBMC culture medium at Day 2 | After 48 hours of culture |
Countries
Canada
Outcome results
None listed