Polyradiculoneuropathy, Chronic Inflammatory Demyelinating
Conditions
Keywords
Plasma exchange, Double filtration plasmapheresis
Brief summary
CIDP, a rare disorder affecting young adults, causes gradual weakness of the limbs, areflexia and impaired sensory function. New CIDP phenotypes without antibodies but with modified cell profiles have been described. Treatments include corticotherapy, IVIg and plasmapheresis but the latter's action mechanisms remain unclear. Plasmapheresis supposedly removes toxic agents like antibodies from plasma but it is uncertain whether it has an immune-modulating effect. Also, the refining mechanisms of the two main plasmapheresis techniques - single plasma exchange and double filtration plasmapheresis (DFPP) - are different and unclear. This study aims to compare the evolution of peripheral lymphocyte profiles in patients with CIDP according to their treatment (single centrifugation plasmapheresis or DFPP) to better grasp the action mechanisms of both techniques.
Interventions
BIOLOGICALBlood sampling
Blood sampling before and after each type of cycle, Single Plasma Exchange or Double Filtration Plasmapheresis
Sponsors
Centre Hospitalier Universitaire de Nīmes
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
TREATMENT
Masking
SINGLE (Subject)
Masking description
Randomisation to one of two treatments before alternation.
Intervention model description
Proof-of-concept, monocentric, prospective, Single-Case Experimental Design
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* Patients with confirmed Chronic Inflammatory Demyelinating Polyneuropathy (CIDP) without pathogenic antibodies and stable under therapeutic plasmapheresis with one session every 2-3 weeks for at least two months. * Patients who have given free written informed consent. * Patients who have signed the consent form. * Patients affiliated to or beneficiaries of a health insurance scheme. * Adult patients (≥18 ans). * Healthy subjects of the same age and sex
Exclusion criteria
* Patients Under the age of 18. * Patients under immunosuppressive treatment for less than 3 months. * Patients participating in Category 1 research involving human subjects. * Patients in an exclusion period determined by another study. * Patients under legal protection, guardianship or curatorship. * Patients unable to express consent. * Patients for whom it is impossible to give informed information. * Pregnant, parturient or breastfeeding patients. * Healthy subject with chronic pathology or drug treatment
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Cycle 5, Group B : TH17 cells 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %. |
| Cycle 5, Group B : TH17 cells AFTER double filtration plasmapheresis | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %. |
| Cycle 5, Group B : TH17 cells BEFORE double filtration plasmapheresis | Day 0, Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH17 cells will be measured in %. |
| Cycle 5, Group B : TH1 cells 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %. |
| Cycle 6, Group B : T-reg cells 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %. |
| Cycle 6, Group B : T-reg cells AFTER single plasma exchange | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %. |
| Cycle 6, Group B : T-reg cells BEFORE single plasma exchange | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of T-reg cells will be measured in %. |
| Cycle 6, Group B : TH17 cells 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %. |
| Cycle 6, Group B : TH17 cells AFTER single plasma exchange | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %. |
| Cycle 6, Group B : TH17 cells BEFORE single plasma exchange | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH17 cells will be measured in %. |
| Cycle 6, Group B : TH1 cells 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %. |
| Cycle 6, Group B : TH1 cells AFTER single plasma exchange | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %. |
| Cycle 6, Group B : TH1 cells BEFORE single plasma exchange | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %. |
| Cycle 6, Group A : T-reg cells 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %. |
| Cycle 5, Group B : TH1 cells AFTER double filtration plasmapheresis | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %. |
| Cycle 6, Group A : T-reg cells AFTER double filtration plasmapheresis | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %. |
| Cycle 6, Group A : T-reg cells BEFORE double filtration plasmapheresis | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of T-reg cells will be measured in %. |
| Cycle 6, Group A : TH17 cells 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %. |
| Cycle 6, Group A : TH17 cells AFTER double filtration plasmapheresis | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %. |
| Cycle 6, Group A : TH17 cells BEFORE double filtration plasmapheresis | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH17 cells will be measured in %. |
| Cycle 6, Group A : TH1 cells 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %. |
| Cycle 6, Group A : TH1 cells AFTER double filtration plasmapheresis | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %. |
| Cycle 6, Group A : TH1 cells BEFORE double filtration plasmapheresis | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %. |
| Cycle 5, Group B : T-reg cells 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %. |
| Cycle 5, Group B : T-reg cells AFTER double filtration plasmapheresis | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %. |
| Cycle 5, Group B : T-reg cells BEFORE double filtration plasmapheresis | Day 0, Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of T-reg cells will be measured in %. |
| Cycle 1, Group A : TH1 cells 7-10 days AFTER single plasma exchange | Day 7 -10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %. |
| Cycle 1, Group A : TH1 cells BEFORE single plasma exchange | Day 0, Time 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %. |
| Cycle 1, Group A : TH1 cells AFTER single plasma exchange | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %. |
| Cycle 1, Group A : TH17 cells BEFORE first single plasma exchange | Day 0, Time 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH17 peripheral lymphocyte cells will be measured in %. |
| Cycle 1, Group A : TH17 cells AFTER single plasma exchange | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 peripheral lymphocyte cells will be measured in %. |
| Cycle 1, Group A : TH17 cells 7 - 10 days AFTER single plasma exchange | Day 7 - 10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 peripheral lymphocyte cells will be measured in %. |
| Cycle 1, Group A : T-reg cells BEFORE first single plasma exchange | Day 0, Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of T-reg cells will be measured in %. |
| Cycle 1, Group A : T-reg cells AFTER single plasma exchange | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %. |
| Cycle 1, Group A : T-reg cells 7 - 10 days AFTER single plasma exchange | Day 7 - 10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %. |
| Cycle 1, Group B : TH1 cells BEFORE first double filtration plasmapheresis | Day 0, Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %. |
| Cycle 1, Group B : TH1 cells AFTER the first double filtration plasmapheresis | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %. |
| Cycle 1, Group B : TH1 cells 7-10 days AFTER the first double filtration plasmapheresis | Day 7 - 10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %. |
| Cycle 1, Group B : TH17 cells BEFORE the first double filtration plasmapheresis | Day0, Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH17 cells will be measured in %. |
| Cycle 1, Group B : TH17 cells AFTER the first double filtration plasmapheresis | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %. |
| Cycle 1, Group B : T17 cells 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %. |
| Cycle 1, Group B : T-reg cells BEFORE double filtration plasmapheresis | Day 0, Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of T-reg cells will be measured in %. |
| Cycle 1, Group B : T-reg cells AFTER double filtration plasmapheresis | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %. |
| Cycle 1, Group B : T-reg cells 7-10 days AFTER double filtration plasmapheresis | Day 7 - 10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %. |
| Cycle 2, Group A: TH1 cells BEFORE double filtration plasmapheresis | Day 0, Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH1 cells will be measured in %. |
| Cycle 2, Group A: TH1 cells AFTER double filtration plasmapheresis | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 cells will be measured in %. |
| Cycle 2, Group A: TH1 cells 7-10 days AFTER double filtration plasmapheresis | Day 7 - 10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 cells will be measured in %. |
| Cycle 2, Group A: TH17 cells BEFORE double filtration plasmapheresis | Day 0, Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH17 cells will be measured in %. |
| Cycle 2, Group A: TH17 cells AFTER double filtration plasmapheresis | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %. |
| Cycle 2, Group A: TH17 cells 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %. |
| Cycle 2, Group A: T-reg cells BEFORE double filtration plasmapheresis | Day 0, Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of T-reg cells will be measured in %. |
| Cycle 2, Group A: T-reg cells AFTER double filtration plasmapheresis | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %. |
| Cycle 2, Group A: T-reg cells 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %. |
| Cycle 2, Group B: TH1 cells BEFORE single plasma exchange | Day 0, Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH1 cells will be measured in %. |
| Cycle 2, Group B: TH1 cells AFTER single plasma exchange | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 cells will be measured in %. |
| Cycle 2, Group B: TH1 cells 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 cells will be measured in %. |
| Cycle 2, Group B: T17 cells BEFORE single plasma exchange | Day 0, Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of T17 cells will be measured in %. |
| Cycle 2, Group B: TH17 cells AFTER single plasma exchange | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T17 cells will be measured in %. |
| Cycle 2, Group B: TH17 cells 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %. |
| Cycle 2, Group B: T-reg cells BEFORE single plasma exchange | Day 0, Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of T-reg cells will be measured in %. |
| Cycle 2, Group B: T-reg cells AFTER single plasma exchange | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %. |
| Cycle 2, Group B: T-reg cells 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %. |
| Cycle 3, Group A: TH1 cells BEFORE single plasma exchange | Day 1, Hour 1 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %. |
| Cycle 3, Group A: TH1 cells AFTER single plasma exchange | Day 1, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %. |
| Cycle 3, Group A: T1 cells 7-10 days AFTER single plasma exchange | Day 7 - 10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %. |
| Cycle 3, Group A: TH17 cells BEFORE single plasma exchange | Day 1, Hour 1 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH17 cells will be measured in %. |
| Cycle 3, Group A: TH17 cells AFTER single plasma exchange | Day 1, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %. |
| Cycle 3, Group A: TH17 cells 7-10 days AFTER single plasma exchange | Day 7 - 10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %. |
| Cycle 3, Group A: T-reg cells BEFORE single plasma exchange | Day 0, Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of T-reg cells will be measured in %. |
| Cycle 3, Group A: T-reg cells AFTER single plasma exchange | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %. |
| Cycle 3, Group A: T-reg cells 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %. |
| Cycle 3, Group B: TH1 cells BEFORE double filtration plasmapheresis | Day 0, Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %. |
| Cycle 3, Group B: TH1 cells AFTER double filtration plasmapheresis | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %. |
| Cycle 3, Group B: TH1 cells 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %. |
| Cycle 3, Group B: TH17 cells BEFORE double filtration plasmapheresis | Day 0, Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH17 cells will be measured in %. |
| Cycle 3, Group B: TH17 cells AFTER double filtration plasmapheresis | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %. |
| Cycle 3, Group B: TH17 cells 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %. |
| Cycle 3, Group B: T-reg cells BEFORE double filtration plasmapheresis | Day 0, Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of T-reg cells will be measured in %. |
| Cycle 3, Group B: T-reg cells AFTER double filtration plasmapheresis | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %. |
| Cycle 3, Group B: T-reg cells 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %. |
| Cycle 4, Group A: T1 cells BEFORE double filtration plasmapheresis | Day 0, Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of T1 peripheral lymphocyte cells will be measured in %. |
| Cycle 4, Group A: T1 cells AFTER double filtration plasmapheresis | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T1 peripheral lymphocyte cells will be measured in %. |
| Cycle 4, Group A: TH1 cells 7-10 days AFTER double filtration plasmapheresis | Day 7 - 10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %. |
| Cycle 4, Group A: TH17 cells BEFORE double filtration plasmapheresis | Day 0, Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH17 cells will be measured in %. |
| Cycle 4, Group A: TH17 cells AFTER double filtration plasmapheresis | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %. |
| Cycle 4, Group A: TH17 cells 7-10 days AFTER double filtration plasmapheresis | Day 7 - 10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 peripheral lymphocyte cells will be measured in %. |
| Cycle 4, Group A: T-reg cells BEFORE double filtration plasmapheresis | Day 0, Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of T-reg cells will be measured in %. |
| Cycle 4, Group A: T-reg cells AFTER double filtration plasmapheresis | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %. |
| Cycle 4, Group A: T-reg cells 7-10 days AFTER double filtration plasmapheresis | Day 7 - 10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg peripheral lymphocyte cells will be measured in %. |
| Cycle 4, Group B : TH1 cells BEFORE single plasma exchange | Day 0, Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %. |
| Cycle 4, Group B : TH1 cells AFTER single plasma exchange | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %. |
| Cycle 4, Group B : TH1 cells 7-10 days AFTER single plasma exchange | Day 7 -10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %. |
| Cycle 4, Group B : TH17 cells BEFORE single plasma exchange | Day 0, Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH17 cells will be measured in %. |
| Cycle 4, Group B : TH17 cells AFTER single plasma exchange | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %. |
| Cycle 4, Group B : T17 cells 7-10 days AFTER single plasma exchange | Day 7 -10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T1 peripheral lymphocyte cells will be measured in %. |
| Cycle 4, Group B : T-reg cells BEFORE single plasma exchange | Day 0, Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of T-reg cells will be measured in %. |
| Cycle 4, Group B : T-reg cells AFTER single plasma exchange | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %. |
| Cycle 4, Group B : T-reg cells 7-10 days AFTER single plasma exchange | Day 7 - 10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %. |
| Cycle 5, Group A : TH1 cells BEFORE single plasma exchange | Day 0, Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %. |
| Cycle 5, Group A : TH1 cells AFTER single plasma exchange | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %. |
| Cycle 5, Group A : TH1 cells 7-10 days AFTER single plasma exchange | Day 7 - 10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 cells will be measured in %. |
| Cycle 5, Group A : TH17 cells BEFORE single plasma exchange | Day 0, Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH17 cells will be measured in %. |
| Cycle 5, Group A : TH17 cells AFTER single plasma exchange | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %. |
| Cycle 5, Group A : TH17 cells 7-10 days AFTER single plasma exchange | Day 7 - 10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %. |
| Cycle 5, Group A : T-reg cells BEFORE single plasma exchange | Day 0, Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of T-reg cells will be measured in %. |
| Cycle 5, Group A : T-reg cells AFTER single plasma exchange | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %. |
| Cycle 5, Group A : T-reg cells 7-10 days AFTER single plasma exchange | Day 7 - 10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %. |
| Cycle 5, Group B : TH1 cells BEFORE double filtration plasmapheresis | Day 0, Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Cycle 2, Group B : CCR6-CXCR3- cells 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 3, Group A : T4 lymphocytes BEFORE single plasma exchange | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 3, Group A : T4 DR+ lymphocytes BEFORE single plasma exchange | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 3, Group A : T8 lymphocytes BEFORE single plasma exchange | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 3, Group A : T8 DR+ lymphocytes BEFORE single plasma exchange | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 3, Group A : B lymphocytes BEFORE single plasma exchange | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 3, Group A : CC45RA+CCR7+ cells BEFORE single plasma exchange | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 3, Group A : CC45RA-CCR7+ cells BEFORE single plasma exchange | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 3, Group A : CC45RA-CCR7- cells BEFORE single plasma exchange | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 3, Group A : CCR6-CXCR3- cells BEFORE single plasma exchange | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 3, Group A : T4 lymphocytes AFTER single plasma exchange | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 3, Group A : T4 DR+ lymphocytes AFTER single plasma exchange | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 3, Group A : T8 lymphocytes AFTER single plasma exchange | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 3, Group A : T8 DR+ lymphocytes AFTER single plasma exchange | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 3, Group A : B lymphocytes AFTER single plasma exchange | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 3, Group A : CD45RA+CCR7+ cells AFTER single plasma exchange | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 3, Group A : CD45RA-CCR7+ cells AFTER single plasma exchange | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 3, Group A : CD45RA-CCR7- cells AFTER single plasma exchange | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 3, Group A : CCR6-CXCR3- cells AFTER single plasma exchange | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 3, Group A : T4 DR+ lymphocytes 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 3, Group A : T4 lymphocytes 7 -10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 3, Group A : T8 lymphocytes 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 3, Group A : T8 DR+ lymphocytes 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 3, Group A : B lymphocytes 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 3, Group A : CD45RA+CCR7+ cells 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 3, Group A : CD45RA-CCR7+ cells 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 3, Group A : CD45RA-CCR7- cells 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 3, Group A : CCR6-CXCR3- cells 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 3, Group B : T4 lymphocytes BEFORE double filtration plasmapheresis | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 3, Group B : T4 DR+ lymphocytes BEFORE double filtration plasmapheresis | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 3, Group B : T8 lymphocytes BEFORE double filtration plasmapheresis | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 3, Group B : T8 DR+ lymphocytes BEFORE double filtration plasmapheresis | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 3, Group B : B lymphocytes BEFORE double filtration plasmapheresis | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 3, Group B : CD45RA+CCR7+ cells BEFORE double filtration plasmapheresis | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 3, Group B : CD45RA-CCR7+ cells BEFORE double filtration plasmapheresis | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 3, Group B : CD45RA-CCR7- cells BEFORE double filtration plasmapheresis | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 3, Group B : CCR6-CXCR3- cells BEFORE double filtration plasmapheresis | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 3, Group B : T4 lymphocytes AFTER double filtration plasmapheresis | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 3, Group B : T4 DR+ lymphocytes AFTER double filtration plasmapheresis | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 3, Group B : T8 lymphocytes AFTER double filtration plasmapheresis | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 3, Group B : T8 DR+ lymphocytes AFTER double filtration plasmapheresis | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 3, Group B : B lymphocytes AFTER double filtration plasmapheresis | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 3, Group B : CD45RA+CCR7+ cells AFTER double filtration plasmapheresis | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 3, Group B : CD45RA-CCR7+ cells AFTER double filtration plasmapheresis | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 3, Group B : CD45RA-CCR7- cells AFTER double filtration plasmapheresis | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 3, Group B : CCR6-CXCR3- cells AFTER double filtration plasmapheresis | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 3, Group B : T4 lymphocytes 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 3, Group B : T4 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 3, Group B : T8 lymphocytes 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 3, Group B : T8 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 3, Group B : B lymphocytes 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 3, Group B : CD45RA+CCR7+ cells 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 3, Group B : CD45RA-CCR7+ cells 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 3, Group B : CD45RA-CCR7- cells 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 3, Group B : CCR6-CXCR3- cells 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 4, Group A : T4 lymphocytes BEFORE double filtration plasmapheresis | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 4, Group A : T4 DR+ lymphocytes BEFORE double filtration plasmapheresis | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 4, Group A : T8 lymphocytes BEFORE double filtration plasmapheresis | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 4, Group A : T8 DR+ lymphocytes BEFORE double filtration plasmapheresis | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 4, Group A : B lymphocytes BEFORE double filtration plasmapheresis | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 4, Group A : CD45RA+CCR7+ cells BEFORE double filtration plasmapheresis | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 4, Group A : CD45RA-CCR7+ cells BEFORE double filtration plasmapheresis | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 4, Group A : CD45RA-CCR7- cells BEFORE double filtration plasmapheresis | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 4, Group A : CCR6-CXCR3- cells BEFORE double filtration plasmapheresis | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 4, Group A : T4 lymphocytes AFTER double filtration plasmapheresis | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 4, Group A : T4 DR+ lymphocytes AFTER double filtration plasmapheresis | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 4, Group A : T8 lymphocytes AFTER double filtration plasmapheresis | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 4, Group A : T8 DR+ lymphocytes AFTER double filtration plasmapheresis | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 4, Group A : B lymphocytes AFTER double filtration plasmapheresis | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 4, Group A : CD45RA+CCR7+ cells AFTER double filtration plasmapheresis | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 4, Group A : CD45RA-CCR7+ cells AFTER double filtration plasmapheresis | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 4, Group A : CD45RA-CCR7- cells AFTER double filtration plasmapheresis | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 4, Group A : CCR6-CXCR3- cells AFTER double filtration plasmapheresis | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 4, Group A : T4 lymphocytes 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 4, Group A : T4 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 4, Group A : T8 lymphocytes 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 4, Group A : T8 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 4, Group A : B lymphocytes 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 4, Group A : CD45RA+CCR7+ cells 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 4, Group A : CD45RA-CCR7+ cells 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 4, Group A : CD45RA-CCR7- cells 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 4, Group A : CCR6-CXCR3- cells 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 4, Group B : T4 lymphocytes BEFORE plasma exchange | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 4, Group B : T4 DR+ lymphocytes BEFORE plasma exchange | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 4, Group B : T8 lymphocytes BEFORE plasma exchange | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 4, Group B : T8 DR+ lymphocytes BEFORE single plasma exchange | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 4, Group B : B lymphocytes BEFORE single plasma exchange | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 4, Group B : CD45RA+CCR7+ cells BEFORE single plasma exchange | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 4, Group B : CD45RA-CCR7+ cells BEFORE single plasma exchange | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 4, Group B : CD45RA-CCR7- cells BEFORE single plasma exchange | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 4, Group B : CCR6-CXCR3- cells BEFORE single plasma exchange | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 4, Group B : T4 lymphocytes AFTER single plasma exchange | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 4, Group B : T4 DR+ lymphocytes AFTER single plasma exchange | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 4, Group B : T8 lymphocytes AFTER single plasma exchange | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 4, Group B : T8 DR+ lymphocytes AFTER single plasma exchange | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 4, Group B : B lymphocytes AFTER single plasma exchange | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 4, Group B : CD45RA+CCR7+ cells AFTER single plasma exchange | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 4, Group B : CD45RA-CCR7+ cells AFTER single plasma exchange | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 4, Group B : CD45RA-CCR7- cells AFTER single plasma exchange | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 4, Group B : CCR6-CXCR3- cells AFTER single plasma exchange | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 4, Group B : T4 lymphocytes 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 4, Group B : T4 DR+ lymphocytes 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 4, Group B : T8 lymphocytes 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 4, Group B : T8 DR+ lymphocytes 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 4, Group B : B lymphocytes 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 4, Group B : CD45RA+CCR7+ cells 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 4, Group B : CD45RA-CCR7+ cells 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 4, Group B : CD45RA-CCR7- cells 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 4, Group B : CCR6-CXCR3- cells 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 5, Group A : T4 lymphocytes BEFORE single plasma exchange | Day 0, Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 5, Group A : T4 DR+ lymphocytes BEFORE single plasma exchange | Day 0, Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 5, Group A : T8 lymphocytes BEFORE single plasma exchange | Day 0, Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 5, Group A : T8 DR+ lymphocytes BEFORE single plasma exchange | Day 0, Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 5, Group A : B lymphocytes BEFORE single plasma exchange | Day 0, Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 5, Group A : CD45RA+CCR7+ cells BEFORE single plasma exchange | Day 0, Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 5, Group A : CD45RA-CCR7+ cells BEFORE single plasma exchange | Day 0, Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 5, Group A : CD45RA-CCR7- cells BEFORE single plasma exchange | Day 0, Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 5, Group A : CCR6-CXCR3- cells BEFORE single plasma exchange | Day 0, Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 5, Group A : T4 lymphocytes AFTER single plasma exchange | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 5, Group A : T4 DR+ lymphocytes AFTER single plasma exchange | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 5, Group A : T8 lymphocytes AFTER single plasma exchange | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 5, Group A : T8 DR+ lymphocytes AFTER single plasma exchange | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 5, Group A : B lymphocytes AFTER single plasma exchange | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 5, Group A : CD45RA+CCR7+ cells AFTER single plasma exchange | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 5, Group A : CD45RA-CCR7+ cells AFTER single plasma exchange | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 5, Group A : CD45RA-CCR7- cells AFTER single plasma exchange | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 5, Group A : CCR6-CXCR3- cells AFTER single plasma exchange | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 5, Group A : T4 lymphocytes 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 5, Group A : T4 DR+ lymphocytes 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 5, Group A : T8 lymphocytes 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 5, Group A : T8 DR+ lymphocytes 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 5, Group A : B lymphocytes 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 5, Group A : CD45RA+CCR7+ cells 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 5, Group A : CD45RA-CCR7+ cells 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 5, Group A : CD45RA-CCR7- cells 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 5, Group A : CCR6-CXCR3- cells 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 5, Group B : T4 lymphocytes BEFORE double filtration plasmapheresis | Day 0, Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 5, Group B : CCR6-CXCR3- cells AFTER double filtration plasmapheresis | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 5, Group B : T4 lymphocytes 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 5, Group B : T4 DR+ lymphocytes BEFORE double filtration plasmapheresis | Day 0, Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 5, Group B : T8 lymphocytes BEFORE double filtration plasmapheresis | Day 0, Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 5, Group B : T8 DR+ lymphocytes BEFORE double filtration plasmapheresis | Day 0, Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 5, Group B : B lymphocytes BEFORE double filtration plasmapheresis | Day 0, Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 5, Group B : CD45RA+CCR7+ cells BEFORE double filtration plasmapheresis | Day 0, Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 5, Group B : CD45RA-CCR7+ cells BEFORE double filtration plasmapheresis | Day 0, Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 5, Group B : CD45RA-CCR7- cells BEFORE double filtration plasmapheresis | Day 0, Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 5, Group B : CCR6-CXCR3- cells BEFORE double filtration plasmapheresis | Day 0, Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 5, Group B : T4 lymphocytes AFTER double filtration plasmapheresis | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 5, Group B : T4 DR+ lymphocytes AFTER double filtration plasmapheresis | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 5, Group B : T8 lymphocytes AFTER double filtration plasmapheresis | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 5, Group B : T8 DR+ lymphocytes AFTER double filtration plasmapheresis | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 5, Group B : B lymphocytes AFTER double filtration plasmapheresis | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 5, Group B : CD45RA+CCR7+ cells AFTER double filtration plasmapheresis | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 5, Group B : CD45RA-CCR7- cells AFTER double filtration plasmapheresis | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 5, Group B : T4 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 5, Group B : T8 lymphocytes 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 5, Group B : T8 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 5, Group B : B lymphocytes 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 5, Group B : CD45RA+CCR7+ cells 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 5, Group B : CD45RA-CCR7+ cells 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 5, Group B : CD45RA-CCR7- cells 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 5, Group B : CCR6-CXCR3- cells 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 6, Group A : T4 lymphocytes BEFORE double filtration plasmapheresis | Day 0, Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 6, Group A : T4 DR+ lymphocytes BEFORE double filtration plasmapheresis | Day 0, Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 6, Group A : T8 lymphocytes BEFORE double filtration plasmapheresis | Day 0, Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 6, Group A : T8 DR+ lymphocytes BEFORE double filtration plasmapheresis | Day 0, Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 6, Group A : B lymphocytes BEFORE double filtration plasmapheresis | Day 0, Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 6, Group A : CD45RA+CCR7+ cells BEFORE double filtration plasmapheresis | Day 0, Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 6, Group A : CD45RA-CCR7+ cells BEFORE double filtration plasmapheresis | Day 0, Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 6, Group A : CD45RA-CCR7- cells BEFORE double filtration plasmapheresis | Day 0, Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 6, Group A : CCR6-CXCR3- cells BEFORE double filtration plasmapheresis | Day 0, Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 6, Group A : T4 lymphocytes AFTER double filtration plasmapheresis | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 6, Group A : T4 DR+ lymphocytes AFTER double filtration plasmapheresis | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 6, Group A : T8 lymphocytes AFTER double filtration plasmapheresis | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 6, Group A : T8 DR+ lymphocytes AFTER double filtration plasmapheresis | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 6, Group A : B lymphocytes AFTER double filtration plasmapheresis | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 6, Group A : CD45RA+CCR7+ cells AFTER double filtration plasmapheresis | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 6, Group A : CD45RA-CCR7+ cells AFTER double filtration plasmapheresis | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 6, Group A : CD45RA-CCR7- cells AFTER double filtration plasmapheresis | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 6, Group A : CCR6-CXCR3- cells AFTER double filtration plasmapheresis | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 6, Group A : T4 lymphocytes 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 6, Group A : T4 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 6, Group A : T8 lymphocytes 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 6, Group A : T8 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 6, Group A : B lymphocytes 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 6, Group A : CD45RA+CCR7+ cells 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 6, Group A : CD45RA-CCR7+ cells 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 6, Group A : CD45RA-CCR7- cells 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 6, Group A : CCR6-CXCR3- cells 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 6, Group B : T4 lymphocytes BEFORE single plasma exchange | Day 0, H0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 6, Group B : T4 DR+ lymphocytes BEFORE single plasma exchange | Day 0, H0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 6, Group B : T8 lymphocytes BEFORE single plasma exchange | Day 0, H0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 6, Group B : T8 DR+ lymphocytes BEFORE single plasma exchange | Day 0, H0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 6, Group B : B lymphocytes BEFORE single plasma exchange | Day 0, H0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 6, Group B : CD45RA+CCR7+ cells BEFORE single plasma exchange | Day 0, H0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 6, Group B : CD45RA-CCR7+ cells BEFORE single plasma exchange | Day 0, H0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 6, Group B : CD45RA-CCR7- cells BEFORE single plasma exchange | Day 0, H0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 6, Group B : T8 lymphocytes 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 6, Group B : CCR6-CXCR3- cells BEFORE single plasma exchange | Day 0, H0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 6, Group B : T4 lymphocytes AFTER single plasma exchange | Day 0, H3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 6, Group B : T4 DR+ lymphocytes AFTER single plasma exchange | Day 0, H3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 6, Group B : T8 lymphocytes AFTER single plasma exchange | Day 0, H3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 6, Group B : T8 DR+ lymphocytes AFTER single plasma exchange | Day 0, H3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 6, Group B : B lymphocytes AFTER single plasma exchange | Day 0, H3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 6, Group B : CD45RA+CCR7+ cells AFTER single plasma exchange | Day 0, H3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 6, Group B : CD45RA-CCR7+ cells AFTER single plasma exchange | Day 0, H3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 6, Group B : CD45RA-CCR7- cells AFTER single plasma exchange | Day 0, H3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 6, Group B : CCR6-CXCR3- cells AFTER single plasma exchange | Day 0, H3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 6, Group B : T4 lymphocytes 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 6, Group B : T4 DR+ lymphocytes 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 6, Group B : T8 DR+ lymphocytes 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 6, Group B : B lymphocytes 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 6, Group B : CD45RA+CCR7+ cells 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 6, Group B : CD45RA-CCR7+ cells 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 6, Group B : CD45RA-CCR7- cells 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 6, Group B : CCR6-CXCR3- cells 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 5, Group B : CD45RA-CCR7+ cells AFTER double filtration plasmapheresis | Day 0, Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 1, Group A : T4 lymphocytes BEFORE single plasma exchange | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 1, Group A : T4-DR+ lymphocytes BEFORE single plasma exchange | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 1, Group A : T8 lymphocytes BEFORE single plasma exchange | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 1, Group A : T8 DR+ lymphocytes BEFORE single plasma exchange | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 1, Group A : B lymphocytes BEFORE single plasma exchange | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 1, Group A : CD45RA+CCR7+ cells BEFORE single plasma exchange | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 1, Group A : CD45RA-CCR7+ cells BEFORE single plasma exchange | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 1, Group A : CD45RA-CCR7- cells BEFORE single plasma exchange | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 1, Group A : CCR6-CXCR3- cells BEFORE single plasma exchange | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 1, Group A : T4 lymphocytes AFTER single plasma exchange | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 1, Group A : T4-DR+ lymphocytes AFTER single plasma exchange | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 1, Group A : T8 lymphocytes AFTER single plasma exchange | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 1, Group A : T8 DR+ lymphocytes AFTER single plasma exchange | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 1, Group A : B lymphocytes AFTER single plasma exchange | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 1, Group A : CD45RA+CCR7+ cells AFTER single plasma exchange | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 1, Group A : CD45RA-CCR7+ cells AFTER single plasma exchange | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 1, Group A : CD45RA-CCR7- cells AFTER single plasma exchange | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 1, Group A : CR6-CXCR3- cells AFTER single plasma exchange | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 1, Group A : T4 lymphocytes 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 1, Group A : T4-DR+ lymphocytes 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 1, Group A : T8 lymphocytes 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 1, Group A : T8 DR+ lymphocytes 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 1, Group A : B lymphocytes 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 1, Group A : CD45RA+CCR7+ cells 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 1, Group A : CD45RA-CCR7+ cells 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 1, Group A : CD45RA-CCR7- cells 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 1, Group A : CCR6-CXCR3- cells 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 1, Group B: T4 lymphocytes BEFORE double filtration plasmapheresis | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 1, Group B: T4 DR+ lymphocytes BEFORE double filtration plasmapheresis | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 1, Group B: T8 lymphocytes BEFORE double filtration plasmapheresis | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 1, Group B: T8 DR+ lymphocytes BEFORE double filtration plasmapheresis | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 1, Group B: B lymphocytes BEFORE double filtration plasmapheresis | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 1, Group B: CD45RA+CCR7+ cells BEFORE double filtration plasmapheresis | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 1, Group B: CD45RA-CCR7+ cells BEFORE double filtration plasmapheresis | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 1, Group B: CD45RA-CCR7- cells BEFORE double filtration plasmapheresis | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 1, Group B: CCR6-CXCR3- cells BEFORE double filtration plasmapheresis | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 1, Group B:T4 lymphocytes AFTER double filtration plasmapheresis | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 1, Group B:T4 DR+ lymphocytes AFTER double filtration plasmapheresis | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 1, Group B:T8 lymphocytes AFTER double filtration plasmapheresis | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 1, Group B:T8 DR+ lymphocytes AFTER double filtration plasmapheresis | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 1, Group B: B lymphocytes AFTER double filtration plasmapheresis | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 1, Group B: CD45RA+CCR7+ cells AFTER double filtration plasmapheresis | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 1, Group B: CD45RA-CCR7+ cells AFTER double filtration plasmapheresis | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 1, Group B: CD45RA-CCR7- cells AFTER double filtration plasmapheresis | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 1, Group B: CCR6-CXCR3- cells AFTER double filtration plasmapheresis | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 1, Group B: T4 lymphocytes 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 1, Group B: T4 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 1, Group B: T8 lymphocytes 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 1, Group B: T8 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 1, Group B: B lymphocytes 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 1, Group B: CD45RA+CCR7+ cells 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 1, Group B: CD45RA-CCR7+ cells 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 1, Group B: CD45RA-CCR7- cells 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 1, Group B: CCR6-CXCR3- cells 7-10 days AFTER double filtration plasmapheresis | Day 7-10 | 5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 2, Group A : T4 lymphocytes BEFORE double filtration plasmapheresis | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 2, Group A : T4 DR+ lymphocytes BEFORE double filtration plasmapheresis | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 2, Group A : T8 lymphocytes BEFORE double filtration plasmapheresis | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 2, Group A : T8 DR+ lymphocytes BEFORE double filtration plasmapheresis | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 2, Group A : B lymphocytes BEFORE double filtration plasmapheresis | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) a,d measured as a percentage. |
| Cycle 2, Group A : CD45RA+CCR7+ cells BEFORE double filtration plasmapheresis | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) a,d measured as a percentage. |
| Cycle 2, Group A : CD45RA-CCR7+ cells BEFORE double filtration plasmapheresis | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) a,d measured as a percentage. |
| Cycle 2, Group A : CD45RA-CCR7- cells BEFORE double filtration plasmapheresis | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) a,d measured as a percentage. |
| Cycle 2, Group A : CCR6-CXCR3- cells BEFORE double filtration plasmapheresis | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) a,d measured as a percentage. |
| Cycle 2, Group A : T4 lymphocytes AFTER double filtration plasmapheresis | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) a,d measured as a percentage. |
| Cycle 2, Group A : T4 DR+ lymphocytes AFTER double filtration plasmapheresis | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 2, Group A : T8 lymphocytes AFTER double filtration plasmapheresis | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 2, Group A : T8 DR+ lymphocytes AFTER double filtration plasmapheresis | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 2, Group A : B lymphocytes AFTER double filtration plasmapheresis | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 2, Group A : CD45RA+CCR7+ cells AFTER double filtration plasmapheresis | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 2, Group A : CD45RA-CCR7+ cells AFTER double filtration plasmapheresis | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 2, Group A : CD45RA-CCR7- cells AFTER double filtration plasmapheresis | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 2, Group A : CCR6-CXCR3- cells AFTER double filtration plasmapheresis | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 2, Group A : T4 lymphocytes 7-10 days AFTER double filtration plasmapheresis | Day 7 - 10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 2, Group A : T4 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis | Day 7 - 10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 2, Group A : T8 lymphocytes 7-10 days AFTER double filtration plasmapheresis | Day 7 - 10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 2, Group A : T8 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis | Day 7 - 10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 2, Group A : B lymphocytes 7-10 days AFTER double filtration plasmapheresis | Day 7 - 10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 2, Group A : CD45RA+CCR7+ cells 7-10 days AFTER double filtration plasmapheresis | Day 7 - 10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 2, Group A : CD45RA-CCR7+ cells 7-10 days AFTER double filtration plasmapheresis | Day 7 - 10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 2, Group A : CD45RA-CCR7- cells 7-10 days AFTER double filtration plasmapheresis | Day 7 - 10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 2, Group A : CCR6-CXCR3- cells 7-10 days AFTER double filtration plasmapheresis | Day 7 - 10 | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 2, Group B : T4 lymphocytes BEFORE single plasma exchange | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 2, Group B : T4 DR+ lymphocytes BEFORE single plasma exchange | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 2, Group B : T8 lymphocytes BEFORE single plasma exchange | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 2, Group B : T8 DR+ lymphocytes BEFORE single plasma exchange | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 2, Group B : B lymphocytes BEFORE single plasma exchange | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 2, Group B : CD45RA+CCR7+ cells BEFORE single plasma exchange | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 2, Group B : CD45RA-CCR7+ cells BEFORE single plasma exchange | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 2, Group B : CD45RA-CCR7- cells BEFORE single plasma exchange | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 2, Group B : CCR6-CXCR3- cells BEFORE single plasma exchange | Day 0 Hour 0 | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 2, Group B : T4 lymphocytes AFTER single plasma exchange | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 2, Group B : T4 DR+ lymphocytes AFTER single plasma exchange | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 2, Group B : T8 lymphocytes AFTER single plasma exchange | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 2, Group B : T8 DR+ lymphocytes AFTER single plasma exchange | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 2, Group B : B lymphocytes AFTER single plasma exchange | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 2, Group B : CD45RA+CCR7+ cells AFTER single plasma exchange | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 2, Group B : CD45RA-CCR7+ cells AFTER single plasma exchange | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 2, Group B : CD45RA-CCR7- cells AFTER single plasma exchange | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 2, Group B : CCR6-CXCR3- cells AFTER single plasma exchange | Day 0 Hour 3 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 2, Group B : T4 lymphocytes 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 2, Group B : T4 DR+ lymphocytes 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 2, Group B : T8 lymphocytes 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 2, Group B : T8 DR+ lymphocytes 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 2, Group B : B lymphocytes 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 2, Group B : CD45RA+CCR7+ cells 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 2, Group B : CD45RA-CCR7+ cells 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Cycle 2, Group B : CD45RA-CCR7- cells 7-10 days AFTER single plasma exchange | Day 7-10 | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured a percentage. |
Other
| Measure | Time frame | Description |
|---|---|---|
| Group A, Cycle 1 : Link between variations in lymphocyte sub-populations and the clinical evolution . | Day 7-10 after the end of Cycle 1 (each cycle lasts 3 hours) | All the above primary and secondary outcome measures will be compared with clinical information in the patient file in order to determine a link between these variations at the end of each treatment cycle. |
| Compare lymphocyte phenotype between patients with PIDC and control subjects. | Day 0 | Significant difference in Th1/Th2 ratio between PIDC patients and control subjects. |
| Constitution of a biological resource centre using the blood samples taken in view of future research, especially immunological phenotyping. | Day 0, Hour 0 | After all the above analyses, the unused aliquots from the samples taken (bottom of tubes) will be conserved and indexed. |
| Group B, Cycle 6 : Cost of double filtration to the health institution. | Day 7-10 after the end of Cycle 6 (each cycle lasts 3 hours) | The cost of each cycle will be recorded in Euros in the electronic clinical research file and the average amount will be calculated from this information at the end of 6 cycles. |
| Group A, Cycle 6 : Cost of double filtration to the health institution. | Day 7-10 after the end of Cycle 6 (each cycle lasts 3 hours) | The cost of each cycle will be recorded in Euros in the electronic clinical research file and the average amount will be calculated from this information at the end of 6 cycles. |
| Group B, Cycle 5 : Cost of double filtration to the health institution. | Day 7-10 after the end of Cycle 5 (each cycle lasts 3 hours) | The cost of each cycle will be recorded in Euros in the electronic clinical research file and the average amount will be calculated from this information at the end of 6 cycles. |
| Group A, Cycle 5 : Cost of single plasma exchange to the health institution. | Day 7-10 after the end of Cycle 5 (each cycle lasts 3 hours) | The cost of each cycle will be recorded in Euros in the electronic clinical research file and the average amount will be calculated from this information at the end of 6 cycles. |
| Group B, Cycle 4 : Cost of single plasma exchange to the health institution. | Day 7-10 after the end of Cycle 4 (each cycle lasts 3 hours) | The cost of each cycle will be recorded in Euros in the electronic clinical research file and the average amount will be calculated from this information at the end of 6 cycles. |
| Group A, Cycle 4 : Cost of double filtration plasmapheresis to the health institution. | Day 7-10 after the end of Cycle 4 (each cycle lasts 3 hours) | The cost of each cycle will be recorded in Euros in the electronic clinical research file and the average amount will be calculated from this information at the end of 6 cycles. |
| Group B, Cycle 3 : Cost of double filtration plasmapheresis to the health institution. | Day 7-10 after the end of Cycle 3 (each cycle lasts 3 hours) | The cost of each cycle will be recorded in Euros in the electronic clinical research file and the average amount will be calculated from this information at the end of 6 cycles. |
| Group A, Cycle 3 : Cost of single plasma exchange to the health institution. | Day 7-10 after the end of Cycle 3 (each cycle lasts 3 hours) | The cost of each cycle will be recorded in Euros in the electronic clinical research file and the average amount will be calculated from this information at the end of 6 cycles. |
| Group B, Cycle 2 : Cost of single plasma exchange to the health institution. | Day 7-10 after the end of Cycle 2 (each cycle lasts 3 hours) | The cost of each cycle will be recorded in Euros in the electronic clinical research file and the average amount will be calculated from this information at the end of 6 cycles. |
| Group A, Cycle 2 : Cost of double filtration plasmapheresis to the health institution. | Day 7-10 after the end of Cycle 2 (each cycle lasts 3 hours) | The cost of each cycle will be recorded in Euros in the electronic clinical research file and the average amount will be calculated from this information at the end of 6 cycles. |
| Group B, Cycle 1 : Cost of double filtration plasmapheresis to the health institution. | Day 7-10 after the end of Cycle 1 (each cycle lasts 3 hours) | The cost of each cycle will be recorded in Euros in the electronic clinical research file and the average amount will be calculated from this information at the end of 6 cycles. |
| Group A, Cycle 1 : Cost of single plasma exchange to the health institution. | Day 7-10 after the end of Cycle 1 (each cycle lasts 3 hours) | The cost of each cycle will be recorded in Euros in the electronic clinical research file and the average amount will be calculated from this information at the end of 6 cycles. |
| Group B, Cycle 6 : Link between variations in lymphocyte sub-populations and the clinical evolution . | Day 7-10 after the end of Cycle 6 (each cycle lasts 3 hours) | All the above primary and secondary outcome measures will be compared with clinical information in the patient file in order to determine a link between these variations at the end of each treatment cycle. |
| Group A, Cycle 6 : Link between variations in lymphocyte sub-populations and the clinical evolution . | Day 7-10 after the end of Cycle 6 (each cycle lasts 3 hours) | All the above primary and secondary outcome measures will be compared with clinical information in the patient file in order to determine a link between these variations at the end of each treatment cycle. |
| Group B, Cycle 5 : Link between variations in lymphocyte sub-populations and the clinical evolution . | Day 7-10 after the end of Cycle 5 (each cycle lasts 3 hours) | All the above primary and secondary outcome measures will be compared with clinical information in the patient file in order to determine a link between these variations at the end of each treatment cycle. |
| Group A, Cycle 5 : Link between variations in lymphocyte sub-populations and the clinical evolution . | Day 7-10 after the end of Cycle 5 (each cycle lasts 3 hours) | All the above primary and secondary outcome measures will be compared with clinical information in the patient file in order to determine a link between these variations at the end of each treatment cycle. |
| Group B, Cycle 4 : Link between variations in lymphocyte sub-populations and the clinical evolution . | Day 7-10 after the end of Cycle 4 (each cycle lasts 3 hours) | All the above primary and secondary outcome measures will be compared with clinical information in the patient file in order to determine a link between these variations at the end of each treatment cycle. |
| Group A, Cycle 4 : Link between variations in lymphocyte sub-populations and the clinical evolution . | Day 7-10 after the end of Cycle 4 (each cycle lasts 3 hours) | All the above primary and secondary outcome measures will be compared with clinical information in the patient file in order to determine a link between these variations at the end of each treatment cycle. |
| Group B, Cycle 3 : Link between variations in lymphocyte sub-populations and the clinical evolution . | Day 7-10 after the end of Cycle 3 (each cycle lasts 3 hours) | All the above primary and secondary outcome measures will be compared with clinical information in the patient file in order to determine a link between these variations at the end of each treatment cycle. |
| Group A, Cycle 3 : Link between variations in lymphocyte sub-populations and the clinical evolution . | Day 7-10 after the end of Cycle 3 (each cycle lasts 3 hours) | All the above primary and secondary outcome measures will be compared with clinical information in the patient file in order to determine a link between these variations at the end of each treatment cycle. |
| Group B, Cycle 2 : Link between variations in lymphocyte sub-populations and the clinical evolution . | Day 7-10 after the end of Cycle 2 (each cycle lasts 3 hours) | All the above primary and secondary outcome measures will be compared with clinical information in the patient file in order to determine a link between these variations at the end of each treatment cycle. |
| Group A, Cycle 2 : Link between variations in lymphocyte sub-populations and the clinical evolution . | Day 7-10 after the end of Cycle 2 (each cycle lasts 3 hours) | All the above primary and secondary outcome measures will be compared with clinical information in the patient file in order to determine a link between these variations at the end of each treatment cycle. |
| Group B, Cycle 1 : Link between variations in lymphocyte sub-populations and the clinical evolution . | Day 7-10 after the end of Cycle 1 (each cycle lasts 3 hours) | All the above primary and secondary outcome measures will be compared with clinical information in the patient file in order to determine a link between these variations at the end of each treatment cycle. |
Countries
France
Outcome results
None listed