Muscle Injury, Muscle Soreness
Conditions
Keywords
DOMS,, Lemon verbena
Brief summary
In the present study, investigators investigated whether supplementation with lemon verbena extract (LVE) could improve muscle damage and biochemical indicators after exhaustive exercise challenge. Based on maximum jump heights, 60 subjects (30 males and 30 females) were equally divided into a placebo group (0 mg/human/day) and an LVE supplement group (400 mg/human/day), with gender-equal distribution. All subjects started supplementation 10 days before exhaustive exercise and continued it until all tests were completed. Before the intervention, after the exhaustive exercise, and on the following 3 days, the participants underwent 12-minute Cooper running/walking; blood collection; assessments of pain, muscle stiffness, maximum jump heights, and isometric maximum muscle strength; and anaerobic strength tests.
Interventions
DIETARY_SUPPLEMENTLvemon verbena extract
Each capsule contained 200 mg of lemon verbena extract 2 capsules/day, 14 days
DIETARY_SUPPLEMENTmaltodextrin
Each capsule contained 0 mg of lemon verbena extract 2 capsules/day, 14 days
Sponsors
National Taiwan Sport University
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
TREATMENT
Masking
TRIPLE (Subject, Caregiver, Investigator)
Eligibility
Sex/Gender
ALL
Age
20 Years to 30 Years
Healthy volunteers
Yes
Inclusion criteria
* health * Must be able to swallow tablets
Exclusion criteria
* non-smokers * no musculoskeletal * medical * metabolic disease * women not on the reproductive cycle * high blood pressure, asthma * skeletal neuromuscular injuries in the upper or lower extremities
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| inflammation of IL-6 level | 10 days | To assess inflammation indicators, volunteers fasted for at least 8 hours before performing muscle exhaustion exercises. At designated time points in the recovery period, including baseline (0) and 3h, 24h, and 48 after exercise, blood samples were collected with brachial venous catheters. Assess serum IL-6 levels (pg/ml) to monitor physiological fitness. Used commercial kit human IL-6 immunoassay (R&D system, Minnesota, USA) and Elisa reader (PerkinElmer, Massachusetts, USA) for analysis |
| Clinical Biochemistry of CK level | 10 days | To assess muscle damage, volunteers fasted for at least 8 hours before performing muscle exhaustion exercises. At designated time points in the recovery period, including baseline (0) and 3h, 24h, and 48 after exercise, blood samples were collected with brachial venous catheters. Serum CK (U/L), were assessed for monitoring physiological adaptation. All biochemical indices were assessed using an autoanalyzer (Hitachi 7060, Tokyo, Japan). |
| Stress hormones 8-Hydroxy-2'-Deoxyguanosine (8-OHdG) in Urine | 10 days | Subjects' urine was collected 24 hours after the exhaustive exercise for analysis. Used commercial kit human 8-OHdG (Cayman, Mich, USA) and Elisa reader (PerkinElmer, Massachusetts, USA) for analysis |
| Assessment of muscle stiffness | 10 days | 3 h, 24h, 48h after exhausting exercise, use Myoton machine to evaluate muscle stiffness (N/m) (Myoton, Tallinn, Estonia) |
| Visual Analogue Scale (VAS) evaluation | 10 days | 3 h, 24h, 48h after exhausting exercise, use VAS questionnaire to record muscle pain. the minimum is 0 and maximum is 10, and whether higher scores mean more pain |
Countries
Taiwan
Outcome results
None listed