Aminoacidemia, Appetitive Behavior
Conditions
Keywords
dietary protein, insect protein, young men, beef collagen, amino acids, appetite
Brief summary
Dietary protein plays an important role in appetite regulation. Source of ingested dietary protein may have different effects on appetite, satiety, and/or food intake in humans. Insects are a rich source of protein consumed by many people around the world; however, the capacity of insect-derived protein to regulate appetite and food intake is unclear. The purpose of this study was to compare postprandial plasma glucose, insulin, amino acid, and appetite regulatory hormone concentrations, subjective appetite sensations, and food intake following the ingestion of 25 g of cricket- and beef-derived protein in healthy young males.
Detailed description
With the rapid increase in the global population, the production of sufficient amounts of conventional animal-based protein to meet global dietary demands may no longer be desired nor feasible. Insects may represent an environmentally sustainable additional source of dietary protein that has the potential to help ensure global food security in the future. However, the functional characteristics of insect-derived proteins when fed to humans is unclear. Further, how insect-derived proteins compare to other animal-derived proteins (e.g. from beef) have not been explored. The purpose of this study was to compare postprandial plasma glucose, insulin, and amino acid concentrations, gut-derived appetite regulatory hormones, subjective appetite sensations, and ad libitum energy intake following the ingestion of 25 g insect- or beef-derived protein in healthy young men. It was hypothesized that hyperaminoacidaemia would be more rapid following the ingestion of beef-derived protein compared to cricket-derived protein, although total amino acid availability would be similar between protein sources over a 300-minute postprandial period. It was further hypothesized there would be no difference between protein sources on postprandial subjective appetite sensations or subsequent ad libitum energy intake.
Interventions
DIETARY_SUPPLEMENTCricket-derived Protein
Beverage containing 25 g cricket-derived protein
DIETARY_SUPPLEMENTBeef-derived Protein
Beverage containing 25 g beef-derived protein
Sponsors
McGill University
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
QUADRUPLE (Subject, Caregiver, Investigator, Outcomes Assessor)
Eligibility
Sex/Gender
MALE
Age
18 Years to 35 Years
Healthy volunteers
Yes
Inclusion criteria
* Male * Aged between 18-35 years inclusive * Healthy, moderately active * BMI \< 30 kg/m2 and \> 18.5 kg/m2 * Having given informed consent
Exclusion criteria
* Presence of any identified metabolic or intestinal disorders * Use of tobacco products * Adherence to a strict vegetarian or vegan diet * Use of medications known to affect protein metabolism * Allergy to shellfish or crustaceans * Allergy to beef protein * Engagement in physical exercise training more than 6 days per week
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Plasma leucine concentration | 0-5 hours in the post-prandial period | umol/L |
| Subjective appetite sensations (hunger, fullness, desire to eat, prospective food consumption) | 0-5 hours in the post-prandial period | mm (visual analogue scale) |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Plasma non-essential amino acid concentration | 0-5 hours in the post-prandial period | umol/L |
| Plasma total amino acid concentration | 0-5 hours in the post-prandial period | umol/L |
| Plasma glucose | 0-5 hours in the post-prandial period | mmol/L |
| Plasma branched-chain amino acid concentration | 0-5 hours in the post-prandial period | umol/L |
| Plasma GLP-1 | 0-5 hours in the post-prandial period | pg/mL |
| Plasma PYY | 0-5 hours in the post-prandial period | pg/mL |
| Ad libitum food energy intake | assessed 5 hours after protein intake | kJ |
| Plasma insulin | 0-5 hours in the post-prandial period | pmol/L |
| Plasma essential amino acid concentration | 0-5 hours in the post-prandial period | umol/L |
Countries
Canada
Outcome results
None listed