Healthy, Hyperlactatemia, Lactate
Conditions
Keywords
Lipolysis, Catabolism, Glucose metabolism, Aminoacid metabolisme
Brief summary
Lactate may have anti-lipolytic effects when plasma concentrations of lactate reach levels similar to those seen during high intensity exercise. This study aims to investigate how lactate concentrations similar to those achieved during high intensity exercise affects lipolysis in humans. In addition to this, to investigate how increased lactate concentrations affects glucose- and amino acid metabolism. 8 healthy males will be included. Study participants will undergo two separate investigation days that will be identical except for the interventions: 1. Intravenous sodium D/L-lactate 2. Intravenous sodium chloride. The study consists of a 3-hour basal period followed by a 3-hour hyperinsulinemic euglycemic clamp. During the study we will: * Estimate insulin sensitivity during the hyperinsulinemic euglycemic clamp (M value) * Use tracer kinetics to estimate lipid-, glucose and amino acid metabolism using \[9,10-3H\]-palmitate, \[3-3H\]-glucose, \[15N\]-phenylalanine, \[15N\]-tyrosine, \[2H4\]-tyrosine and \[13C\]-Urea. * Do muscle- and adipose tissue biopsies for analyses of signaling pathways involved in regulation of lipid-, glucose and amino acid metabolism. * Do blood samples of relevant hormones, metabolites and cytokines. * Use indirect calorimetry to estimate study participants' resting energy expenditure and respiratory quotient during the basal period. * Estimate cardiac ejection fraction by echocardiography and measure blood pressure during both the basal- and clamp period.
Interventions
DRUGNa-lactate
intravenous
DRUGSodium chloride
Intravenous
Sponsors
University of Aarhus
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
OTHER
Masking
SINGLE (Subject)
Eligibility
Sex/Gender
MALE
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
* male * \>18 years of age * BMI \>19 and below 30 * Written and verbal consent to participation
Exclusion criteria
* Chronic illness * daily use of medicine * affected blood samples at screening, as assessed by the PI * does not speak and understand Danish * Assessed unsuitable by PI.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Acute Metabolic effects of Na-lactate compared with NaCl on lipolysis | 2 hours (1 hour basal period, 1 hour clamp) | Change in \[9,10-3H\]-palmitate tracer flux |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Acute Metabolic effects of Na-lactate compared with NaCl on glucose metabolism | 6 hours (3 hours basal period, 3 hours clamp) | Change in glucose metabolism quantified with \[3-3H\]-glucose tracer |
| Insulin sensitivity | 3 hours. | Hyperinsulinaemic euglycaemic clamp with 0.6mU/kg/min insulin and 20% glucose to calculate M value. Difference between interventions. |
| Acute metabolic effects of Na-lactate compared with NaCl on aminoacid metabolism | 6 hours | Change in aminoacid metabolism quantified with \[15N\]-phenylalanine, \[15N\]tyrosine, \[2H4\]tyrosine and \[13C\]-Urea tracers. |
| Indirect calorimetry during clamp | 20 minutes | Change in estimation of resting energy expenditure during clamp. Difference between interventions. |
| Indirect calorimetry during basal conditions | 20 minutes | Change in estimation of respiratory quotient during basal period. Difference between interventions. |
| Echocardiography | 15 minutes | Change in cardiac ejection fraction during basal period. Difference between interventions. |
| blood pressure | 2 x 10 min | blood pressure (systolic and diastolic) during basal- and clamp |
| Blood samples - Insulin | 6 hours. | Insulin concentrations. Difference between interventions. |
| Changes in signaling in muscle tissue between interventions | After 3 and 5 hours of intervention | Western blot examinations of muscle tissue biopsies |
| Blood samples - glucagon | 6 hours. | glucagon concentrations. Difference between interventions. |
| Blood samples - cortisol | 6 hours. | cortisol concentrations. Difference between interventions. |
| Blood samples - Ghrelin | 6 hours. | Ghrelin concentrations. Difference between interventions. |
| Blood samples - GDF-15 | 6 hours. | GDF-15 concentrations. Difference between interventions. |
| Blood samples - free fatte acids | 6 hours. | Free fatte acids concentrations. Difference between interventions. |
| Blood samples - glucose | 6 hours. | glucose concentrations. Difference between interventions. |
| Blood samples - lactate | 6 hours. | lactate concentrations. Difference between interventions. |
| Changes in signaling in adipose tissue between interventions | After 3 and 5 hours of intervention | Western blot examinations of adipose tissue biopsies |
| Blood samples - c-peptid | 6 hours. | c-peptid concentrations. Difference between interventions. |
Countries
Denmark
Outcome results
None listed