Covid19
Conditions
Brief summary
The investigators will study nasopharunx microbiota composition, RNA sequences and in vitro cytokines production by peripheral blood cells of COVID-19 patients (both mild-moderate and severe cases) (comparing with convalescent patients and healthy controls).
Detailed description
The investigators will compare study parameters (basal) between mild-moderate and severe COVID-19 patients and with other study groups (i.e. convalescent and healthy controls). Nasopharunx microbiota dysbiosis, in vitro cytokines production profile, and peripheral blood flocytometric results of the four study groups will be compared. In vitro cytokines production will be studied and compared in Nill (No antigens) and Mitojen (Cocktail of antigens) pre-prepared test tubes.
Interventions
OTHERObservation
The study parameters will be obtained at the time of recuirement (Basal)
Sponsors
Başakşehir Çam & Sakura City Hospital
Study design
Observational model
CASE_CONTROL
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Age
18 Years to 90 Years
Healthy volunteers
Yes
Inclusion criteria
* Ability to give a written consent
Exclusion criteria
* In ability to give a written consent * Using of medications that affect the results of study parameters * Presence of malignancy or tuberculosis
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Determination of peripheral blood RNA microarray assay | First day | Four study groups, microarray Analysis . |
| Determination of nasopharunx Microbiota analysis | 1st day | Four study groups, metagenomic analysis, to determine abundance and decrease of some species. |
| Determination of in vitro cytokines production | First day | IL-2, IL-7, IL-10, granulocyte-colony stimulating factor, interferon-p inducible protein 10, monocyte chemo-attracting protein 1, macrophage inflammatory protein 1-α and tumor necrosis factor-α in Nill (no angijen) and Mitojen (Cocktails of antigens)tubes (between 4 study groups) |
| Determination of peripheral blood cytometry | First day | Four study groups, peripheral blood cytometry; CD3, CD4, CD8, and CD45 rates. |
| Relation between study parameters | First day parameters | determining the realtion between microbiotme dysbiosis, in vitro cytokines production and peripheral leymphocytes (CD3,CD4, CD8, and CD45) rates and the severity of COVID-19( if any) |
Countries
Turkey (Türkiye)
Contacts
Primary ContactMehmet Hursitoglu, MD
Outcome results
None listed