Diabetic Retinopathy, Glaucoma, Proliferative Vitreoretinopathy, Pterygium
Conditions
Brief summary
The surgically-harvested eye tissue (ie. vitreous-retinal proliferative membrane, outflow pathway, retinas, and pterygium) is a complex tissue responsible for maintaining intraocular homeostasis or mediating ocular pathogenesis. Dysfunction of one or more resident cell types within the tissues results in different ocular disorder, leading to vision loss, or even blindness. In this study, we aim to use single-cell RNA sequencing to generate a comprehensive cell atlas of surgically-harvested eye tissues.
Interventions
PROCEDUREocular surgery
surgery to remove the pathological eye tissue
Sponsors
The First Affiliated Hospital with Nanjing Medical University
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to 90 Years
Healthy volunteers
No
Inclusion criteria
1. Patients with ocular diseases and require surgery intervention. 2. The surgery intervention remove pathological tissue during surgery. 3. Patients consent to the treatment and single-cell sequencing.
Exclusion criteria
1\. Surgically removed eye tissue cannot meet with the quality control of single-cell sequencing (mainly the tissue weight and cell counts);
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| counts of each cell type | 1 month | single-cell sequencing identify what cells and the counts within the eye tissue |
Countries
China
Contacts
Primary ContactZizong Hu, PhD, MD
Outcome results
None listed