Intestinal Bacteria Flora Disturbance
Conditions
Brief summary
In order to understand the life history of the breast milk flora, explore its composition, source of strains, and ability to colonize the baby's intestinal environment, to find out strains with the potential of probiotics in breast milk
Detailed description
This is a double-blind and randomized study. After being discharged from the hospital, the subject was notified to consume the Babyguard breast milk probiotic daily for 1 month, and observe whether the mother's stool, breast milk and baby stool samples can find the same strain. The clinical diagnosis items of the bacterial strains of mother feces, breast milk and baby feces samples are evaluated by the doctor.
Interventions
DIETARY_SUPPLEMENTPlacebo
consume 1 sachet per day for 1 months
DIETARY_SUPPLEMENTBabyguard® Breast Milk Probiotics
consume 1 sachet per day for 1 months
Sponsors
TCI Co., Ltd.
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
TREATMENT
Masking
DOUBLE (Subject, Investigator)
Eligibility
Sex/Gender
FEMALE
Age
20 Years to 65 Years
Healthy volunteers
Yes
Inclusion criteria
* At least 20 years old, pregnant women with non-high-risk pregnancy risk assessed by obstetricians and gynecologists, healthy mothers and infants with no abnormal obstetric examination results
Exclusion criteria
* Pregnant women who have used oral or injectable antibiotics within three months before delivery, or women who need a cesarean section for any reason during pregnancy
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Microflora of mother's feces | 4-6 weeks after the baby is born | Collect mother's feces for bacterial analysis.The mother's feces use Qiagen stool mini kit to extract DNA, and then use the intestinal bacteria primers to detect the presence of intestinal bacteria in the feces. |
| Group. B Streptococcus (GBS) screening | 35-37 weeks of pregnancy | Use sterile cotton sticks for vaginal samples, and then perform Group. B Streptococcus (GBS) screening |
| The microflora of maternal blood | 4-6 weeks after the baby is born | Use venous blood to collect 20 ml of mother's blood for bacterial analysis.The mother's blood uses Qiagen DNA isolation mini kit to extract DNA, and then uses intestinal bacteria primers to detect the presence of intestinal bacteria in the blood |
| Metabolic analysis of maternal urine | 4-6 weeks after the baby is born | Collect 10 ml of mother's urine for intestinal dysbiosis analysis. Use Qiagen DNA isolation mini kit to extract DNA from the mother's urine, and then use the intestinal bacteria primer to detect the presence of intestinal bacteria in the urine. |
| Bacteria analysis of breast milk | 4-6 weeks after the baby is born | Collect 10 ml of breast milk for bacterial phase analysis.Use Qiagen DNA isolation mini kit to extract DNA from breast milk, and then use intestinal bacteria primers to detect whether the breast milk contains intestinal bacteria. |
| Bacteria analysis of infant feces | 4-6 weeks after the baby is born | Collect baby feces for bacterial phase analysis.Use Baby feces use Qiagen stool mini kit to extract DNA, and then use intestinal bacteria primers to detect the presence of intestinal bacteria in the feces. |
Countries
Taiwan
Outcome results
None listed