Ovarian Tissue Cryopreservation, Vitrification
Conditions
Brief summary
Ovarian tissue cryopreservation (OTC) is a new approach on fertility preservation for young female patients with cancer. It is now used by many IVF centers in the world. However, this is the first study which OTC by vitrification is carried on and assessed the effectiveness of the technique.
Detailed description
Ovaries from all less than 45 year-old breast cancer patients who undergo oophorectomy by laparoscopy after treated with chemotherapy will be enrolled to the study. Pieces of cortex of each patient will divide into two groups. Group 1 is fresh fragments (control group) and group 2 is vitrified group. Ovarian tissues in group 2 will be vitrified or warmed with medium and devices of Ova-kit type M (Kitazato, Japan). Cortex fragments of each group will have: * Follicle survival evaluation by Neutral red staining * Histological evaluation by Hematoxylin and Eosin staining * Evaluation relative Growth Differentiation Factor 9 (GDF-9) and Caspase-3 gene expression by real-time Polymerase chain reaction (PCR). Comparison of data collected from 2 groups will be carried on to evaluate the effectiveness of the technique before applying this technique for cancer patients in Vietnam.
Interventions
OTHERevaluation of the effectiveness of vitrification technique on ovarian tissue quality
there are 3 types of experiments in each group, including Follicle survival evaluation by Neutral red staining, Histological evaluation by Hematoxylin and Eosin staining, 2.4. Evaluation GDF-9 and Caspase-3 gene expression by real-time PCR.
Sponsors
Mỹ Đức Hospital
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
OTHER
Masking
NONE
Eligibility
Sex/Gender
FEMALE
Age
No minimum to 45 Years
Healthy volunteers
No
Inclusion criteria
* Ovaries from all less than 45 year-old breast cancer patients who undergo oophorectomy by laparoscopy after treated with chemotherapy
Exclusion criteria
* The volume of ovaries is less than 2/3 of normal 3cm (L) x 2.5cm (H) x 1.5cm (W) ovary.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Rate of Survivable follicle | After 4 hours stained by Neutral red | Percentage of survivable follicles per counted follicles in one fragment |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Rate of Healthy follicle by histological analysis | Immediately after tissue stained by Hematoxylin and Eosin | Healthy follicles of primordial, primary, secondary follicles will be counted in one slide |
| The relative expression ratio ( R ) of genes in follicles | Immediately after the intervention | Follicles in ovarian tissue will be collected, complementary deoxyribonucleic acid (cDNA) synthesis after messenger ribonucleic acid (mRNA) purification, relative quantification PCR for detecting gene expression |
Countries
Vietnam
Contacts
Primary ContactLan N Nguyen, MSC
Backup ContactDang Q Vinh, MD, MCE
Outcome results
None listed