Obesity, Wolman Disease, Nutrition
Conditions
Brief summary
Investigators will in a crossover study test the health effects of eating a dairy-based protein-rich breakfast, isocaloric carbohydrate-rich breakfast or no breakfast in young overweight women. Determination of satiety and hunger using visual analog scores (VAS), collection of blood samples and 2x 24 hours food logs will be collected.
Detailed description
This study is a single-blind randomized Cross-Over trial with 30 women aged 18-30 years, each completing three trials. Participants will consume either dairy-based protein-rich breakfast, isocaloric, fiber matched, fat matched, carbohydrate-rich breakfast or no breakfast in randomized order. Three hours later a standardized ad libitum lunch will be served. Blood samples will be collected (pre, 10 min, 30 min, 60 min, 120 min, 175 min after breakfast) and VAS rating hunger, satiety and desire to eat will be determined (pre, during breakfast, 10, 30, 60, 90, 120, 180 min, during lunch, after lunch). Twenty-four hour food logs on the day before the test day and on the test day will be collected. Participants will be characterized by determining height (cm), weight (kg), and body composition by a dual-energy X-ray (DXA) scan before the first test day.
Interventions
OTHERProtein-rich breakfast
High protein yoghurt containing approx. 10 g protein per 100 g. Participants will be asked to consume 300 g (=3 dl) yoghurt with 40 g oats for breakfast. 1 glas of water (150 ml)
OTHERCarbohydrate-rich breakfast
Low protein breakfast containing approx. 2 g protein per 100 g. Participants will be asked to consume \ 60 g bread, 20 g jam and 250 ml juice for breakfast. 1 glas of water (150 ml)
OTHERNo breakfast
1 glas of water (150 ml)
Sponsors
The Danish Dairy Research Foundation, Denmark
University of Aarhus
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
PREVENTION
Masking
SINGLE (Subject)
Intervention model description
Single-blind randomized crossover trail with three intervention meals.
Eligibility
Sex/Gender
FEMALE
Age
18 Years to 30 Years
Healthy volunteers
Yes
Inclusion criteria
* Body mass index \> 25 * Age 18-25 years * Regular exercise \< 5 hour per week
Exclusion criteria
* illness and use of medication affecting the study outcomes * allergy towards lactose or gluten * weightloss/gain \>5kg the last 6 months * dieting * eating disorder * pregnancy * breast feeding * unable to speak and understand danish
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Energy consumption on test days | 0-24 hours | Total energy consumption on test days (24 hour food log) |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Change in leptin | 0-3 hours | in blood sample |
| Change in insulin | 0-3 hours | in blood sample |
| Change in glucagon | 0-3 hours | in blood sample |
| Change in glucose | 0-3 hours | in blood sample |
| Energy consumption the day before a test day (food registration) | 0-24 hours | Total energy consumption on the day before the test day |
| Change in hunger | 0-3 hours | Visual Analog Scale (10 cm) score for feeling of hunger |
| Change in satiety | 0-3 hours | Visual Analog Scale (10 cm) score for feeling of satiety |
| change in desire | 0-3 hours | Visual Analog Scale (10 cm) score for feeling of desire to eat |
| Change in ghrelin | 0-3 hours | in blood sample |
| Change in glucose-dependent insulinotropic polypeptide (GIP) | 0-3 hours | in blood sample |
| Change in Peptide YY (PYY3-36) | 0-3 hours | in blood sample |
Other
| Measure | Time frame | Description |
|---|---|---|
| Body composition fat | Baseline | DXA-scanning to determine fat mass |
| Body composition fat free | Baseline | DXA-scanning to determine fat free mass |
| Height | Baseline | Height in cm |
| Weight | Baseline | Weight in kg |
Countries
Denmark
Outcome results
None listed