Diet, Healthy
Conditions
Keywords
broccoli sprout, microbiome, cruciferous vegetable
Brief summary
The purpose of this project is to study the molecular and metabolic profiles in human plasma, urine and microbiome composition following the consumption of broccoli sprouts.
Detailed description
The investigators wish to examine metabolic and molecular markers found in blood and in urine after a single dose of sprouts, and evaluate the importance of the gut microbiome on these parameters. Some subjects will receive broccoli sprouts that are grown in deuterium oxide, a commonly used non-toxic, non-radioactive label for vegetables (Tang, Qin et al. 2005), so researchers can identify metabolites in blood and urine that are directly from consumed sprouts. The investigators plan to feed subjects a standardized breakfast and either broccoli sprouts or alfalfa sprouts (control) and look at the presence of metabolites and activity in the blood and urine 3, 6, 24, 48 and 72 hours following intake as well as changes in microbiome composition.
Interventions
OTHERBroccoli sprouts
Subjects will consume one serving (about 1 cup) of broccoli sprouts with breakfast (bagel, cream cheese, and orange juice).
OTHERDeuterium oxide-labelled broccoli sprouts
Subjects will consume one serving (about 1 cup) of deuterium oxide-labelled broccoli sprouts with breakfast (bagel, cream cheese, and orange juice).
OTHERAlfalfa sprouts
Subjects will consume one serving (about 1 cup) of alfalfa sprouts with breakfast (bagel, cream cheese, and orange juice).
OTHERDeuterium oxide-labelled alfalfa sprouts
Subjects will consume one serving (about 1 cup) of deuterium oxide-labelled alfalfa sprouts with breakfast (bagel, cream cheese, and orange juice).
Sponsors
Oregon State University
United States Department of Agriculture (USDA)
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
SINGLE (Subject)
Masking description
participant is blinded to the presence or absence of deuterium label
Eligibility
Sex/Gender
ALL
Age
18 Years to 60 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy men and women, 18-60 years of age * Willing to stop taking herbal and phytochemical (plant-based extract or phytochemical) supplements for 1 week prior to and during the study * Willing to stop cruciferous vegetable and probiotic supplement, food and beverage intake 1 week prior to and during the study * Willing to complete a 10-day food diary during the study * Must be able to give written informed consent
Exclusion criteria
* Body Mass Index (BMI) \<18.5 or \>30.0 kg/m2 * Tobacco use, including e-cigarettes, or smoking of any substance (e.g. cannabis) in the past three months * Pregnancy, breastfeeding, or planning to become pregnant before completing the study * Engaging in vigorous exercise more than 7 hours per week * Have a significant acute or chronic illness such as cardiovascular disease, kidney or liver disease, diabetes, thyroid disorder, or radiation or chemotherapy treatment for cancer within the past five years. * Use of medications to control cholesterol (e.g. statins, cholestyramine) or fat absorption (e.g. orlistat) * Have had bariatric surgery (e.g. gastric bypass, gastric banding, sleeve gastrectomy, etc.), other gastrointestinal procedure (e.g. cholecystectomy) or disorders (e.g. Crohn's disease, celiac disease, ulcerative colitis) * Use of oral antibiotic medication within the 6 months
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Urine sulforaphane | Baseline, 3, 6, 24, 48 and 72 hours after consuming a single serving of sprouts | Change in levels of sulforaphane metabolites in urine |
| Urine sulforaphane metabolites | Baseline, 3, 6, 24, 48 and 72 hours after consuming a single serving of sprouts | Change in levels of sulforaphane metabolites in urine |
| Plasma sulforaphane | Baseline, 3, 6, 24, 48 and 72 hours after consuming a single serving of sprouts | Change in levels of sulforaphane in plasma |
| Plasma sulforaphane metabolites | Baseline, 3, 6, 24, 48 and 72 hours after consuming a single serving of sprouts | Change in levels of sulforaphane metabolites in plasma |
| Stool sample based gut microbiome composition using 16S rRNA gene sequencing | Baseline, 3, 6, 24, 48 and 72 hours after consuming a single serving of sprouts | Change in stool sample based gut microbiome composition using 16S rRNA gene sequencing |
Countries
United States
Contacts
PRINCIPAL_INVESTIGATOREmily Ho, PhD
Oregon State University
Outcome results
None listed