Sepsis
Conditions
Keywords
NLRP3 inflammasome, platelets, cytokines, thrombus, septic shock, sepsis
Brief summary
septic shock is the first cause of mortality in intensive care unit. Innate immunity is the body's first line of defense against pathogens
Detailed description
since their discovery, inflammasomes have an important role during inflammatory response following an aggression. There are intracytoplasmic multiprotein complex activated by cellular stress or infections and is responsible for the release of pro-inflammatory cytokines, including IL-1β. One study focused on the role of the NLRP3 inflammasome in monocyte during sepsis has shown its early alteration that is correlated with mortality. Most studies have analysed inflammasomes in nucleated cells, nevertheless, little is known about inflammasomes in platelets. The aim of the study is to analyze the activation of NLRP3 inflammasome in platelets and leukocytes during sepsis, from patients hospitalized in intensive care unit compare to a control group, until its resolution to better understand the pathophysiology of sepsis
Interventions
OTHERBlood collection
Blood collection (at admission, two days, one week and at discharge) to analyze the activation of NLRP3 inflammasome in platelets and leukocytes
Sponsors
University Hospital, Toulouse
Study design
Observational model
OTHER
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
SEPSIS GROUP: Inclusion Criteria: * hospitalized in intensive care unit with central catheter * age \> or = 18 years * patients suffering from sepsis * hospitalized from less than72 hours
Exclusion criteria
* patients with curatorship or guardianship * pregnant women * patients suffering from malignant blood disease * patients suffering from disease associated with NLRP3 inflammasome activation CONTROL GROUP: Inclusion Criteria: * hospitalized in intensive care unit with central catheter for other reason than infection * age \> or = 18 years
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Activation kinetics of the NLRP3 inflammasome during sepsis until its resolution | From day 0 to the hospital discharge, an average of 1 month | Activation kinetics of the NLRP3 inflammasome from day 0 to patient discharge measured by the area under curve |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Rate of inflammasome NLRP3 activation | Day 0 | Rate of inflammasome NLRP3 activation (measured in blood samples) |
| Severity score measured by Sequential Organ Failure Assessment (SOFA) score | Day 0 | Severity score measured by Sequential Organ Failure Assessment (SOFA) score, from 0 to 4; higher score mean worse outcome |
| Severity score measured by Simplified Gravity Index 2 | Day 0 | Severity score measured by Simplified Gravity Index 2, score from 0 to 163, higher score mean worse outcome |
Countries
France
Outcome results
None listed