Healthy
Conditions
Keywords
Neurophysiology, Psychophysics, Sensory thresholds, Neurons, Pain, neuropathic
Brief summary
Also known as a moving itch, knismesis is a mildly annoying sensation caused by a light movement on the skin, such as from a crawling insect. Its underlying mechanism rely on spatial summation, i.e. amplification of the signal due to summation of stimulated fields, like it is for dynamic allodynia in neuropathic pain. This physiological study aims at determining the physical factors of the cutaneous stimulation inducing knismesis as well as the effect of confounding factors. The main area of exploration will be the back, because of its size and the possibility to blind the participant. The information collected, along with animal data in surrogate models, would help to better understand neuropathic pain in the future.
Detailed description
This is a physiological proof-of-concept, also aiming to validate a functional exploration model in healthy volunteers. Each participant will undergo a battery if psychometric then psychophysics tests, presented sequentially. However, most of the main study tests (knismesis) will be admistered in a random order (crossover). The primary endpoint is to characterise, in the healthy human, the physical determinants of the cutaneous stimulation inducing knismesis: length of the pathway, speed, direction, intensity, continuity break of the contact, hairiness, body site, or distraction by other cutaneous stimuli. The secondary endpoint is to study the relationship between the individual sensitivity to spatial summation (knismesis) and other individual sensitivities or profiles.
Interventions
OTHERBattery of neurophysiological explorations
Visit 1: Biometry collection: gender, age, weight, height, eye colour. * Questionnaires : the State and Trait Anxiety Inventory and the Pain Sensitivity * Threshold of unpleasant light brightness, mechanical sensitivity threshold (von Frey hair). * Training to estimate unpleasantness Under nociceptive and tickling stimuli, to use the handgrip. * Mechanical pain threshold (electronic von Frey), heat pain threshold (Medoc Pathway). * 48 stimulations applied to the back skin, following a predetermined plan with 12 different pathways, imbricated with 4 different speeds of movement. Visit 2 (1 to 8 days later) : * 42 stimulations applied to the back skin, to test the effect of stimulation intensity, the side of application, continuity break of the contact, and distraction by other cutaneous stimuli. The stimuli will follow the best paradigm as identified at the previous visit. * 12 stimulations applied to forearm, cheeks, lower limbs, also testing the effect of hairiness.
Sponsors
NEURO-DOL (UMR 1107 INSERM / UCA)
University Hospital, Clermont-Ferrand
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to 50 Years
Healthy volunteers
Yes
Inclusion criteria
\- Ability to answer French questionnaires. · BMI between 19 and 30 kg/m2 at inclusion.
Exclusion criteria
* Pregnancy or breastfeeding. * Natural intolerance to tickling. * Any cutaneous, ocular or neurological disease contraindicating the psychophysical tests. * Any disease or medical history that could expose the subject to unacceptable risk during the study. * Any medication intake within 7 days before testing. * History of drug or alcohol abuse. * Involvement in another concomitant research protocol. * Major dorsal hairiness. * Inability to undergo the tests. * Legal protection. * Concomitant involvement in another clinical research. * Refusal to participate.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Intensity of annoying sensation | Day 1 | After training, the annoying sensation is expressed by the subject through a handgrip dynamometer (Biopac systems). The signal will be treated and converted into area-under-curve response (Dualé C et al., Reg Anesth Pain Med 2011; 36:110-115). |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Muscular aversive response. | Day 1, Day 8 | The muscular aversive response will be assessed by surface electromyography through paraspinal contact electrodes (Biopac systems). |
Countries
France
Contacts
Primary ContactLise Laclautre
Outcome results
None listed