Genetic Predisposition to Disease, Intervertebral Disc Degeneration
Conditions
Keywords
biobank, functional genomics, transcriptomics, glycomics
Brief summary
The main aim of this study is to establish disease-oriented biobank to facilitate research in biology of the lumbar disc degeneration. Diverse biological samples (whole blood, plasma, disc tissue) along with MRI imaging, clinical, socio-demographic and various omics data (e.g. genomic and transcriptomic) will be available for researchers and clinicians for a variety of further multi-omics studies. It will lay the groundwork for the development of early diagnostics of DDD and its personalized treatment. The study is planned to enroll up to 1,100 patients with different grades of lumbar disc degeneration. It is planned to recruit patients within 36 months.
Interventions
OTHERNon-interventional
Translational, observational study
Sponsors
Institute of Cytology and Genetics
Novosibirsk Research Institute of Traumatology and Orthopaedics n.a. Ya.L. Tsivyan
N.N. Priorov National Medical Research Center of Traumatology and Orthopedics
Novosibirsk State University
Study design
Observational model
OTHER
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to 75 Years
Inclusion criteria
1. Age over 18; 2. Presence of lumbar MRI scans; 3. Signed informed consent for voluntary participation is provided.
Exclusion criteria
1. Any contraindication or inability to undergo baseline procedures; 2. Prior surgeries at any level of the lumbar spine; 3. Other non-degenerative spinal conditions that may have an impact on subject safety, wellbeing or the intent and conduction of the study; 4. History or presence of HIV, hepatitis B, hepatitis C.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Genotyping | Through 3 years | DNA will be extracted from the whole blood samples according to the standard protocol |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Phenotype of patients with lumbar disc degeneration | Through 3 years | General information about a patient, his medical history and MRI scans of lumbar spine |
| Total plasma proteins N-glycosylation profiling | Through 3 years | The plasma glycans profiling will be performed according to standard protocol |
| Total RNA profiling | Through 3 years | The intraoperative material will be homogenized and total RNA will be extracted and converted to cDNA using Kit for the isolation of total RNA and microRNA from cells and tissues and M-MuLV-RH First Strand cDNA Synthesis Kit respectively. The amount of extracted RNA and its quality will be estimated using Bioanalyzer 2100. |
Countries
Russia
Outcome results
None listed