Skeletal Muscle, Extracellular Matrix, Amino Acids Metabolism, Exercise, Dietary Supplements
Conditions
Keywords
Muscle Collagen Turnover, Myofibrillar Protein Synthesis, Extracellular Matrix, Anabolism Signaling, Resistance Exercise, Skeletal Muscle, Bone Turnover
Brief summary
The purpose of this study is to assess which amino acids are affecting acute muscle collagen synthesis in response to supplementation and exercise.
Detailed description
This is a double-blinded, randomized controlled, parallel design study, with 3 treatment groups (placebo, collagen peptide, and essential amino acid). The study will involve 3 visits: a baseline testing/familiarization session, and 2 exercise trials which will include muscle biopsies, blood draws, and the infusion of a tracer isotope in order to measure muscle protein synthesis.
Interventions
DIETARY_SUPPLEMENTEssential Amino Acid
Immediately following the exercise intervention participants will consume their assigned essential amino acid supplement
DIETARY_SUPPLEMENTCollagen Peptide
Immediately following the exercise intervention participants will consume their assigned collagen peptide supplement
DIETARY_SUPPLEMENTMaltodextrin Placebo
Immediately following the exercise intervention participants will consume their assigned maltodextrin supplement
Sponsors
University of British Columbia
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
TRIPLE (Subject, Investigator, Outcomes Assessor)
Intervention model description
\- Double-blinded, randomized controlled, parallel design study, with 3 treatment groups (placebo, collagen peptide, and essential amino acid)
Eligibility
Sex/Gender
MALE
Age
18 Years to 35 Years
Healthy volunteers
Yes
Inclusion criteria
* Can fluently read and write in English * Male * 18 - 35 years
Exclusion criteria
* Diabetes, cardiovascular disease, or current pharmaceutical therapy * Medical conditions impacting ability to undertake strenuous physical activity * Lower body resistance training in the previous 6 months * BMI \> 30 * Currently smoking with cigarettes * Allergy to any study supplements * Currently taking any protein, amino acid, or collagen supplement * Participate in greater than 2 hours of weekly vigorous exercise
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Fractional synthetic rate (change over time) of Myofibrillar Protein Synthesis | 0 - 4 hours post-exercise | Fraction of the myofibrillar enriched protein from muscle biopsy per unit time. |
| Fractional synthetic rate (change over time) Muscle Collagen Synthesis | 0 - 4 hours post-exercise | Fraction of the intramuscular collagen enriched protein from muscle biopsy per unit time. |
| Pro-Collagen Type I N-Propeptide (PINP) | 1 hour prior to exercise | Osteoblastic marker of bone collagen synthesis |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Plasma Amino Acids Response to Supplementation | 0 - 4 hours post-exercise | Amino acid plasma concentrations |
| Pro-Collagen Type III N-Propeptide (PIIINP) | 1 hour prior to exercise | Marker of collagen synthesis |
| Muscle mechanistic target of rapamycin Pathway Activation | 0 - 4 hours post-exercise | Measuring the fold change in enzymes associated with the anabolic signalling of the mTOR pathway (e.g. phosphoinositide 3-kinases (PI3k), protein kinase B (Akt)) |
| PCR Gene Expression of Muscle Collagen Genes (Types I, III, IV) | 0 - 4 hours post-exercise | Measuring the fold change when the collagen genes of interest become detectable in the PCR analysis |
Countries
Canada
Outcome results
None listed