Diabetes, Metabolic Syndrome, NAFLD, Overweight and Obesity
Conditions
Brief summary
The purpose of this study is to investigate dietary compositions effect on liverfat measured by magnetic resonance imaging.
Detailed description
Overweight but otherwise healthy men will be included in the study which is a randomized diet intervention study in a cross over design. The study involves 2 intervention periods of 5 days each, during which the participant ingests two different types of experimental diet. Participants will in randomized order (determined by block randomization) ingest a diet with low carbohydrate and high fat content, and a diet with high carbohydrate and low fat content.
Interventions
OTHERHigh Carb Low Fat Diet
The participants will be randomized to a diet of low carb/ high fat og high carb/low fat
OTHERLow Carb High Fat Diet
The participants will be randomized to a diet of low carb/ high fat og high carb/low fat
Sponsors
University of Copenhagen
Danish Research Centre for Magnetic Resonance
Hvidovre University Hospital
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
NONE
Intervention model description
Randomized diet intervention study in a cross over design
Eligibility
Sex/Gender
MALE
Age
18 Years to 50 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy men being 18-50 years old. * Overweight (BMI 25-35) * Low to moderate physical activity level, defined as ≤ 1 weekly session of aerobic exercise. * Non-smokers * No use of medicine
Exclusion criteria
* Fasting blood glucose indicating overt diabetes (≥7 mmol/l) * HbA1C \>48 mmol/mol * Fasting plasma triacylglycerol indicating dyslipidemia (≥2.2 mmol/l) * FIB-4 score \>1.45 * Contraindications for MR-scan: abdominal height exceed limitations of the MR-scanner, pacemaker or other electronic device implanted, implanted metal devices, severe claustrophobia.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Liverfat | (MRS Day -1 (before intervention starts), Day 5 (after diet intervention is completed) | The effect of high-carbohydrate/low-fat vs low-carbohydrate/high-fat diet on change in MRS determined liver fat. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Glycogen | (MRS Day -1 (before intervention starts), Day 5 (after diet intervention is completed) | effect of the low-carbohydrate/high-fat intake and high-carbohydrate/low-fat intake on hepatic glycogen content measured by 7T 13C-MRS |
| fasting hepatic glucose output | (Day 0 and Day 6 of the diet intervention) | measured by Ra of glucose= (infusion rate of 6,6 2H2-glucose)/TTR |
| fasting blood triacylglycerol concentration | (Day 0 and Day 6 of the diet intervention) | — |
| Insulin clearance determined during steady state insulin infusion | (Day 0 and Day 6 of the diet intervention) | — |
Countries
Denmark
Outcome results
None listed