Telomere Length
Conditions
Keywords
PGT-A, telomere length, cumulus cells, polar body
Brief summary
To investigate whether telomere length (TL) of the first Polar body (PB) correlates with TL in Cumulus cells (CC)
Detailed description
With the present study we want to analyze, as a primary objective, whether telomere length (TL) in the first Polar Body (PB) correlates with TL in the corresponding Cumulus cells (CC). As a secondary objective, a possible correlation between TL in PBs and blastocyst TL and ploidy will be evaluated. To our best knowledge, there are no studies evaluating TL in CC and TL in PBs of the corresponding oocyte. With the present prospective study, we sought to investigate whether there is a correlation between CC-TL and PB-TL. If a correlation exists, CC-TL assessment would serve as a valuable non-invasive technique to gain information about oocyte competence.
Interventions
GENETICFirst polar body biopsy in MII oocytes
Since PBs are by-products of the meiotic division of the oocyte and are not required for fertilization and subsequent embryo development, they can be removed to assess exclusively maternal chromosomal information without harming the embryo integrity. As previously described, PB biopsy does not impact the morphokinetic parameters of the embryo development and can be safely applied without the risk of impairing the reproductive potential of the embryo.
GENETICPGT-A blastocyst
preimplantation genetic screening for aneuploidies
Sponsors
ART Fertility Clinics LLC
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
DIAGNOSTIC
Masking
NONE
Eligibility
Sex/Gender
FEMALE
Age
18 Years to 43 Years
Healthy volunteers
Yes
Inclusion criteria
* BMI 18- 30kg/m2 * Expected normo/high responders * Normal female/male karyotype * Antagonist protocol with agonist trigger. * PGT-A: NGS in blastocysts * Fresh autologous ejaculates (≥5 mill/ml) * Primary and secondary infertility * Only ICSI as insemination technique
Exclusion criteria
• PCOS patients according to International evidence-based guideline for the assessment and management of polycystic ovarian syndrome 2018.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| telomere length (TL) of the first Polar body (PB) and TL in Cumulus cells (CC) | 8 weeks | Correlation between telomere length (TL) of the first Polar body (PB) and TL in Cumulus cells (CC) |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| To correlate TL in male WBC and TL in sperm | 8 weeks | TL will be assess in leukocytes (male´s blood sample) and in the sperm (fresh ejaculate) |
| To correlate TL in female WBC and TL in CC | 8 weeks | TL will be assess in leukocytes (female´s blood sample) and in the cumulus cells (CC) from the retrieved oocytes. |
| To evaluate a possible correlation between TL-CC and TL-TE | 8 weeks | Correlation between TL-CC and TL in trophectoderm biopsy performed in the developed blastocysts |
| To evaluate a possible correlation between TL-CC and ploidy TE | 8 weeks | Correlation between TL-CC and PGT-A results in trophectoderm biopsy performed in the developed blastocysts |
| To correlate TL between both polar bodies | 8 weeks | If the MII fertilize after performing ICSI, polar body biopsy for the second PB will be performed |
| To evaluate FSH/LH gene expression in CC | 8 weeks | During the follicular phase LH whose receptors (LHR) are expressed in granulosa cells under the influence of FSH, plays an important role in the regulation of ovarian function and is essential to promote the growth of the dominant follicle, final oocyte maturation as well as ovulation induction |
| To evaluate a possible correlation between ploidy in PBs and ploidy in TE biopsy | 8 weeks | PGT-A in PB and PGT-A in TE biopsy |
| PB biopsy and embryo development | 8 weeks | To validate that PB biopsy does not impair embryo development compared to their sibling embryos with no PB biopsied |
| To evaluate TL in sperm + TL-PB and TL in TE | 8 weeks | TL in sperm + TL-PB and TL in the resulting biopsied |
| To evaluate telomerase activity (TA) in CC and a possible correlation with CC-TL | 8 weeks | Telomerase is a ribonucleoprotein complex responsible for de novo telomere synthesis and addition of telomeric repeats to existing telomeres |
Countries
United Arab Emirates
Contacts
Primary ContactAna Arnanz Poyatos, MSc
Backup ContactNeelke DeMunck, PhD
Outcome results
None listed