Aging, Pituitary
Conditions
Brief summary
The purpose of this study to age-related differences to the response of a combined pituitary stimulation test with TRH, LHRH and ACTH.
Detailed description
Participants will be recruited in three different age intervals: 18-45 years, 55-80 years and 80+ years. These participants will all undergo the same stimulation test with 0.1 mg LHRH, 0.2 mg TRH, and 0.25 mg ACTH (Synachten). The tests will be perfomed at the same time of day for all particpants (between 08:15 and 08:45) and participants will be fasting for the test. Blood samples will be taken just before and at 10, 20, 30, 45, 60, 90 and 120 minutes following the stimulation test. Serum from blood samples will be stored at -20 degrees until analysis.
Interventions
DIAGNOSTIC_TESTPituitary stimulation test
Pituitary stimulation with 0.1 mg LHRH, 0.2 mg TRH and 0.25 mg ACTH
Sponsors
Aalborg University Hospital
Study design
Observational model
OTHER
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
* Ages between 18-45 or above 55. * Healthy Volunteers
Exclusion criteria
* Medication or conditions affecting pituitary response (such as hormonal birth control, steriod use, thyroid disorders) * Pregnancy or birth within the last year * inability to give informed consent
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Differences in area under the curve in TSH. | Immediately after procedure. | Differences will be measured by calculating the area under the curve adjusted for the basal level for the time of the test period. |
| Differences in area under the curve in Prolactin. | Immediately after procedure. | Differences will be measured by calculating the area under the curve adjusted for the basal level for the time of the test period. |
| Differences in area under the curve in LH. | Immediately after procedure. | Differences will be measured by calculating the area under the curve adjusted for the basal level for the time of the test period. |
| Differences in area under the curve in FSH. | Immediately after procedure. | Differences will be measured by calculating the area under the curve adjusted for the basal level for the time of the test period. |
| Differences in area under the curve in Cortisol. | Immediately after procedure. | Differences will be measured by calculating the area under the curve adjusted for the basal level for the time of the test period. |
Other
| Measure | Time frame | Description |
|---|---|---|
| Differences in time to peak for TSH. | Immediately after procedure. | Delayed responses will be examined by inspecting curves and by comparing area under the curve at the different blood sampling time points during the test. |
| Differences in time to peak for Prolactin. | Immediately after procedure. | Delayed responses will be examined by inspecting curves and by comparing area under the curve at the different blood sampling time points during the test. |
| Differences in peak values in TSH | Immediately after procedure. | Both absolute peak values and peak values relative to basal level will be calculated. |
| Differences in time to peak for FSH. | Immediately after procedure. | Delayed responses will be examined by inspecting curves and by comparing area under the curve at the different blood sampling time points during the test. |
| Differences in time to peak for Cortisol. | Immediately after procedure. | Delayed responses will be examined by inspecting curves and by comparing area under the curve at the different blood sampling time points during the test. |
| Differences in time to peak for LH. | Immediately after procedure. | Delayed responses will be examined by inspecting curves and by comparing area under the curve at the different blood sampling time points during the test. |
| Differences in peak values in Prolactin | Immediately after procedure. | Both absolute peak values and peak values relative to basal level will be calculated. |
| Differences in peak values in LH | Immediately after procedure. | Both absolute peak values and peak values relative to basal level will be calculated. |
| Differences in peak values in FSH | Immediately after procedure. | Both absolute peak values and peak values relative to basal level will be calculated. |
| Differences in peak values in Cortisol | Immediately after procedure. | Both absolute peak values and peak values relative to basal level will be calculated. |
Countries
Denmark
Outcome results
None listed