Metabolism in Adipose Tissue
Conditions
Brief summary
Low volume sprint exercise training has been shown to reduce body fat despite small total energy expenditure (for ref see Gillen and Gibala 2014). IL-6 has been shown to increase adipose tissue lipolysis in vitro. Therefore, in an initial study on effects of sprint exercise on adipose tissue metabolism the investigators will examine acute short-term exercise effects on adipose tissue IL-6 exchange.
Detailed description
PURPOSE: Low volume sprint exercise training has been shown to reduce body fat despite small total energy expenditure (for ref see Gillen and Gibala 2014). IL-6 has been shown to increase adipose tissue lipolysis in vitro. Therefore, in an initial study on effects of sprint exercise on adipose tissue metabolism the investigators examined acute short-term effects on adipose tissue IL-6 exchange. METHODS: Four female and four male subjects perform repeated sprint exercise (3 x 30s with 20 min rest between; Wingate-test). Blood samples are repeatedly obtained, up to 120 min after the last sprint (9 min post ex), from catheters inserted percutaneously into brachial artery and a superficial subcutaneous vein on the anterior abdominal wall providing access to the venous drainage from the subcutaneous adipose tissue and analyzed for IL-6 by ELISA and lactate by spectrophotometry. Fat biopsies from the stomach will be obtained before, 15 min and 120 min after the last sprint. Blood flow will be determined by administration of Xenon-133 in the adipose tissue of the stomach. The release rate of Xenon-133 will be registrated continuously during the total investigation. SUPPORT: This study is supported by grants from the Swedish National Center for Research in Sports
Interventions
OTHERRepeated 30-s sprint exercise
Analyze of Interleukin-6 concentration before and after repeated 30-s sprint exercise
Sponsors
Karolinska Institutet
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
20 Years to 40 Years
Healthy volunteers
Yes
Inclusion criteria
* Physical active * Healthy
Exclusion criteria
* Smokers * Pregnant * On medication * No infection * No asthma
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Il-6 secretion in adipose tissue before and after 30-s repeated sprint exercise | Before 30-s repeated sprint exercise | Stomach fat biopsies and arterial and stomach vein blood are obtained |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Changes in blood flow in adipose tissue | Before 30-s repeated sprint exercise | Xenon-133 is administered by a subcutaneous injection into the abdominal adipose tissue of approximately 1-2 MBq of Xenon-133 in the form of gas, at rest before work. |
| Changes in different metabolites | Before 30-s repeated sprint exercise | Analysed for lactate, ammonia and IL-6 |
Countries
Sweden
Outcome results
None listed