Lymphedema
Conditions
Brief summary
The correlation between oxidative stress and increased lymphedematous limb volume lacks supporting evidence. Lymphedema patients are prone to cellulitis. This study aims to investigate the link between oxidative stress, limb volume, and cellulitis after supermicrosurgical lymphaticovenous anastomosis (LVA).
Detailed description
In this study, we focused on the changes in serum antioxidant biomarkers before and after LVA, detected by isobaric tags for relative and absolute quantitation (iTRAQ)-based quantitative proteomic analysis, enzyme-linked immunosorbent assay (ELISA), and Oxidative Stress Panel Kit. For clinical correlation, magnetic resonance (MR) volumetry was implemented for precise lymphedematous limb volume measurements. Correlations were made between changes in oxidative stress, limb volume, and cellulitis before and after LVA.
Interventions
OTHERpre-LVA
Venous serum samples were collected from patients before LVA.
OTHERpost-LVA
Venous serum samples were collected from patients one-month after LVA.
Sponsors
Chang Gung Memorial Hospital
Study design
Observational model
CASE_CONTROL
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
20 Years to 100 Years
Healthy volunteers
No
Inclusion criteria
* patients with lower limb lymphedema confirmed with lymphoscintigraphy * unilateral lower limb lymphedema * lymphedema duration \> 2 years * no active infection * no prophylactic antibiotic use for at least one month before LVA * no tumor recurrence or metastasis * no consumption of antioxidants, such as vitamin E or ascorbic acid
Exclusion criteria
* Patients with upper limb and bilateral lower limb lymphedema
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| the correlation between post-LVA volume reduction | 6 months after surgery | The primary outcome was determined whether post-LVA volume reduction \< 40% |
Countries
Taiwan
Outcome results
None listed