Enterocolitis, Necrotizing, Gastrointestinal Disease, Prematurity
Conditions
Brief summary
Despite a greater understanding of NEC physiopathology, modest progress has been done in terms of intervention and prevention of the disease over the past three decades, being the mortality rate unchanged. Investigators intend to leverage our knowledge and technical expertise developed with fetal enteroids to further investigate the processes leading to NEC by deriving and performing functional studies on human intestinal enteroids generated from intestinal resection for therapeutic reasons in NEC and non-NEC patients 1. Generate a tissue biorepository composed of: enteroids and other lamina propria cells 2. Comparative studies of the gene expression profile of tissue, epithelial enteroids and underlying lamina propria of NEC, non-NEC, hypoxic and non-hypoxic infants 3. In vitro functional studies for the evaluation of critical factors in NEC pathophysiology 4. In vitro functional studies to identify the activation of processes leading to intestinal epithelium necroptosis and/or apoptosis in bacteria challenged and hypoxic conditions 5. Correlative studies of the impact of perinatal variables on the intestinal barrier functionality at baseline and challenged with pathogens 6. In vitro comparison of the intestinal barrier functionality in infants complicated by condition of prenatal hypoxia versus non hypoxic infants 7. Validation the NEC enteroids as an in vitro model for the identification of treatments and prevention of NEC
Interventions
OTHEROrganoids creation
Organoids are created from discarded tissue
Sponsors
Massachusetts General Hospital
Fondazione IRCCS Ca' Granda, Ospedale Maggiore Policlinico
Study design
Observational model
CASE_CONTROL
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
No minimum to 44 Weeks
Healthy volunteers
No
Inclusion criteria
* Infants, with a postconceptional age below 44 weeks of gestation, undergoing a clinically indicated intervention of partial intestinal resection while hospitalized at the NICU of the Fondazione IRCCS Cà Granda Ospedale Maggiore Policlinico.
Exclusion criteria
* Infants presenting a devastating damage of the intestine
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Comparative studies of gene expression | 2 years | assess the gene expression profile of tissue, epithelial enteroids and underlying lamina propria derived from NEC, non-NEC (further classified as hypoxic and non-hypoxic infants). NB: the unit of analysis will be the organoids derived from patients' tissues Investigators expect to be able to derive 1 cell line per patient, and the number of derived organoids will depend from the viability of individual cell lines. |
| functional studies barrier functionality | 2 years | evaluation of barrier functionality at the baseline and in enteroids-derived monolayers challenged with pathogens, dead bacteria (as postbiotics), LPS, pharmacological agents, enteral nutrients and to evaluate innate immune response and barrier functionality as previously investigated in fetal enteroids and the contribution of myofibroblast, immune and ENS to the immune response |
| functional studies on cellular death | 2 years | studies to identify the activation of processes leading to intestinal epithelium necroptosis and/or apoptosis in bacteria challenged and hypoxic conditions |
| Correlative studies of the impact of perinatal variables | 2 years | Assess how the perinatal features (expression of the neonatal phenotype, as IUGR, chorionamnionitis, perinatal hypoxia) on the intestinal barrier functionality at baseline and challenged with pathogens |
| compare the intestinal barrier functionality in pathological conditions | 2 years | comparison of the intestinal barrier functionality in infants complicated by condition of prenatal hypoxia versus non hypoxic infants |
| Validation of the enteroid NEC model | 2 years | Validate the NEC enteroids as an in vitro model for the identification of treatments and prevention of NEC. |
Contacts
Primary ContactPaola Roggero, MD PHD
Backup Contactvalentina bozzetti, md phd
Outcome results
None listed