Pulp Inflammation
Conditions
Keywords
Therapies, Caspase 3, Immunohistochemistry
Brief summary
This randomized, controlled, blinded clinical trial evaluated the effectiveness of different desensitizing protocols in preventing pulp inflammation after bleaching treatment with 35% hydrogen peroxide (PH) (Whiteness PH 35%).
Detailed description
Thirty healthy third human molars extracted by orthodontic indication were randomized and allocated into five groups (n = 6): CN (negative control): without intervention; CP (Positive control): PH; PMB: PH + photobiomodulation with low power laser; CPP: PH + casein phosphopeptide phosphopeptide amorphous calcium phosphate (CPP-ACPF); NANO: PH + nanohydroxyapatite. The office whitening was performed in two sessions with a single application of 45 min and an interval of 48 hours between them. Pulp tissues were removed for immunohistochemical analysis. Immunostaining was performed for Caspase 3 using an Axio Scope.A1 microscope.
Interventions
OTHERWhiteness HP 35%
Single application of 45 minutes on the buccal surfaces of third molars, with an average thickness of 1 mm
DEVICEWhiteness HP 35% + LASER
A dose of LASER (60 J / cm2, for 16 seconds) was applied at two points, one point in the cervical region and another in the center of the buccal surface of the third molars, using visible infrared LASER therapy (Photon III / DMC, São Carlos, SP, Brazil), with a wavelength of 808 nm, and AsGaAl, as an active medium.
OTHERWhiteness HP 35% + CPP
Topical application of casein phosphopeptide amorphous calcium phosphate fluoride CPP-ACPF (My Paste Plus, Recaldent, GC, USA) with the aid of a microbrush (KG Sorensen, SP, Brazil) in a passive way for 5 minutes.
OTHERWhiteness HP 35% + NANO
A topical application of nanohydroxyapatite (Nano P FGM, SC, Brazil) was performed with the aid of a microbrush (KG Sorensen, SP, Brazil) in a passive way for 5 minutes after teeth whitening.
Sponsors
Universidade Federal do Para
Study design
Allocation
RANDOMIZED
Intervention model
SEQUENTIAL
Primary purpose
TREATMENT
Masking
SINGLE (Outcomes Assessor)
Eligibility
Sex/Gender
ALL
Age
20 Years to 26 Years
Healthy volunteers
Yes
Inclusion criteria
* Lower third molars class I position A * Maxillary third molars horizontal position A * Absence of caries and enamel fracture * Absence of periodontal disease * Extraction of third molars for orthodontic reasons * No allergy to the anesthetic and positive pulp sensitivity test
Exclusion criteria
* Calcification in the middle third of the root * Drug users or smokers * Patients who have already done whitening * Root apex with incomplete formation and patients with systemic problems that would make surgery impossible
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Pulp inflammation | 3 days After extraction of third molars | Evaluation of pulp inflammation through immunohistochemistry of the third molar whitening or not |
Countries
Brazil
Outcome results
None listed